Characteristic Metabolic Changes in Skeletal Muscle due to Vibrio vulnificus Infection in a Wound Infection Model

• Material Type: 博士論文
• Title: Characteristic Metabolic Changes in Skeletal Muscle due to Vibrio vulnificus Infection in a Wound Infection Model
• Author/Editor: Ishida, Kai; Shimohata, Takaaki; Kanda, Yuna; Nguyen, Anh Quoc; Masuda, Rumiko; Yamazaki, Kohei; Uebanso, Takashi; Mawatari, Kazuaki; Kashimoto, Takashige; Takahashi, Akira
• Author Heading: Ishida, Kai; Shimohata, Takaaki; Kanda, Yuna; Nguyen, Anh Quoc; Masuda, Rumiko; Yamazaki, Kohei; Uebanso, Takashi; Mawatari, Kazuaki; Kashimoto, Takashige; Takahashi, Akira
• Publication, Distribution, etc.: American Society for Microbiology
• Publication Date: 2023-03-20
• Publication Date (W3CDTF): 2023-03-20
• Alternative Title: Vibrio vulnificus創傷感染モデルマウスにおける骨格筋内の特徴的な代謝変化; Metabolic Changing in V. vulnificus-Infected Tissue
• Degree grantor/type: 徳島大学
• Date Granted: 2023-03-24
• Date Granted (W3CDTF): 2023-03-24
• Dissertation Number: 甲第3734号
• Degree Type: 博士（栄養学）
• Text Language Code: eng
• Subject Heading: Vibrio vulnificus; CE-TOFMS; host metabolomics; TCA cycle; MARTX toxin
• Target Audience: 一般
• Note (General): Vibrio vulnificus is a bacterium that inhabits warm seawater or brackish water environments and causes foodborne diseases and wound infections. In severe cases, V. vulnificus invades the skeletal muscle tissue, where bacterial proliferation leads to septicemia and necrotizing fasciitis with high mortality. Despite this characteristic, information on metabolic changes in tissue infected with V. vulnificus is not available. Here, we elucidated the metabolic changes in V. vulnificus-infected mouse skeletal muscle using capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS). Metabolome analysis revealed changes in muscle catabolites and energy metabolites during V. vulnificus infection. In particular, succinic acid accumulated but fumaric acid decreased in the infected muscle. However, the virulence factor deletion mutant revealed that changes in metabolites and bacterial proliferation were abolished in skeletal muscle infected with a multifunctional-autoprocessing repeats-in-toxin (MARTX) mutant. On the other hand, mice that were immunosuppressed via cyclophosphamide (CPA) treatment exhibited a similar level of bacterial counts and metabolites between the wild type and MARTX mutant. Therefore, our data indicate that V. vulnificus induces metabolic changes in mouse skeletal muscle and proliferates by using the MARTX toxin to evade the host immune system. This study indicates a new correlation between V. vulnificus infections and metabolic changes that lead to severe reactions or damage to host skeletal muscle.
• Persistent ID (NDL): info:ndljp/pid/12911919; https://dl.ndl.go.jp/pid/12911919
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2023-07-08T03:42:33+09:00
• Format (IMT): application/pdf
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• Periodical Title (URI): http://repo.lib.tokushima-u.ac.jp/118290
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp