Proximal deposition of collagen IV by fibroblasts contributes to basement membrane formation by colon epithelial cells in vitro

• Material Type: 博士論文
• Title: Proximal deposition of collagen IV by fibroblasts contributes to basement membrane formation by colon epithelial cells in vitro
• Author/Editor: Tentaku, Aya; Kurisu, Shusaku; Sejima, Kurumi; Nagao, Toshiki; Takahashi, Akira; Yonemura, Shigenobu
• Author Heading: Tentaku, Aya; Kurisu, Shusaku; Sejima, Kurumi; Nagao, Toshiki; Takahashi, Akira; Yonemura, Shigenobu
• Publication, Distribution, etc.: Federation of European Biochemical Societies|Wiley
• Publication Date: 2022-06-22
• Publication Date (W3CDTF): 2022-06-22
• Alternative Title: 結腸上皮細胞のin vitro基底膜形成における近傍線維芽細胞由来IV型コラーゲンの寄与; BM collagen IV deposition by proximal fibroblasts
• Degree grantor/type: 徳島大学
• Date Granted: 2022-09-22
• Date Granted (W3CDTF): 2022-09-22
• Dissertation Number: 甲第3657号
• Degree Type: 博士（栄養学）
• Text Language Code: eng
• Subject Heading: Basement membrane; collagen IV; fibroblast; colon epithelium; co-culture
• Target Audience: 一般
• Note (General): The basement membrane (BM) underlying epithelial tissue is a thin layer of extracellular matrix that governs tissue integrity and function. Epithelial BMs are generally assembled using BM components secreted from two origins: epithelium and stroma. Although de novo BM formation involves self-assembly processes of large proteins, it remains unclear how stroma-derived macromolecules are transported and assembled, specifically in the BM region. In this study, we established an in vitro co-culture model of BM formation in which DLD-1 human colon epithelial cells were cultured on top of collagen I gel containing human embryonic OUMS-36T-2 fibroblasts as stromal cells. A distinct feature of our system is represented by OUMS-36T-2 cells which are almost exclusively responsible for synthesis of collagen IV, a major BM component. Exploiting this advantage, we found that collagen IV incorporation was significantly impaired in culture conditions where OUMS-36T-2 cells were not allowed to directly contact DLD-1 cells. Soluble collagen IV, once diluted in the culture medium, did not accumulate in the BM region efficiently. Live imaging of fluorescently tagged collagen IV revealed that OUMS-36T-2 cells deposited collagen IV aggregates directly onto the basal surface of DLD-1 cells. Collectively, these results indicate a novel mode of collagen IV deposition in which fibroblasts proximal to epithelial cells exclusively contribute to collagen IV assembly during BM formation.
• Persistent ID (NDL): info:ndljp/pid/12970397; https://dl.ndl.go.jp/pid/12970397
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2023-08-04T23:03:35+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
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• Periodical Title (URI): http://repo.lib.tokushima-u.ac.jp/117543
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp