Alternative TitleThe functions of dermatan sulphate in stem cells.
Note (General)Mouse embryonic stem cells (mESCs) have self-renewal and pluripotency. Various signals and growth factors maintain undifferentiated state and regulate differentiation of mESCs. Glycosylation is a one of the post-translational modifications. Glycans have various structures and are conjugated to proteins and lipids. Many proteins on the cell surface and secreted proteins are glycosylated and contribute to important biological phenomena. The signal regulation is one of them, in which signal ligands and receptors bind to specific glycan structures. Previously, we and other groups reported that heparan sulphate, one of glycosaminoglycans, contributes to maintenance of undifferentiated state and regulation of mESCs differentiation. It has been shown that chondroitin sulfate is needed for pluripotency and differentiation of mESCs, while keratan sulfate is a known marker of human ESCs / iPSCs. However, the function of dermatan sulphate (DS) in mESCs has not been fully elucidated. Here, we performed (1) induction of neuronal differentiation in mESCs and human neural stem cells adding purified DS and (2) knockdown or overexpression of the dermatan-4-O-sulfotransferase-1 (D4ST1) in mESCs. We revealed that (1) DS promotes neuronal differentiation in both mouse and human stem cells and (2) D4ST1 contributes to the undifferentiated state of mESCs.
Collection (particular)国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文
Date Accepted (W3CDTF)2023-10-11T15:41:03+09:00
Data Provider (Database)国立国会図書館 : 国立国会図書館デジタルコレクション