A bifurcation concept for B-lymphoid/plasmacytoid dendritic cells with largely fluctuating transcriptome dynamics

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A bifurcation concept for B-lymphoid/plasmacytoid dendritic cells with largely fluctuating transcriptome dynamics

Persistent ID (NDL): info:ndljp/pid/13024219

Material type: 博士論文

Author: Nagaharu, Keiki

Publisher: 三重大学

Publication date: 2022-12-21

Material Format: Digital

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Name of awarding university/degree: 三重大学,博士(医学)

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Note (General)：: application/pdfHematopoiesis was considered a hierarchical stepwise process but was revised to a continuous process following single-cell RNA sequenci...

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Material Type: 博士論文
Title: A bifurcation concept for B-lymphoid/plasmacytoid dendritic cells with largely fluctuating transcriptome dynamics
Author/Editor: Nagaharu, Keiki
Author Heading: Nagaharu, Keiki
Publication, Distribution, etc.: 三重大学
Publication Date: 2022-12-21
Publication Date (W3CDTF): 2022-12-21
Degree grantor/type: 三重大学
Date Granted: 2022-12-21
Date Granted (W3CDTF): 2022-12-21
Dissertation Number: 甲医学第2165号
Degree Type: 博士(医学)
Text Language Code: eng
Alias of Author: ナガハル, ケイキ
永春, 圭規
Target Audience: 一般
Note (General): application/pdf
Hematopoiesis was considered a hierarchical stepwise process but was revised to a continuous process following single-cell RNA sequencing. However, the uncertainty or fluctuation of single-cell transcriptome dynamics during differentiation was not considered, and the dendritic cell (DC) pathway in the lymphoid context remains unclear. Here, we identify human B-plasmacytoid DC (pDC) bifurcation as large fluctuating transcriptome dynamics in the putative B/NK progenitor region by dry and wet methods. By converting splicing kinetics intodiffusiondynamics in a deepgenerativemodel,our original computationalmethodology reveals strongfluctuation at B/pDC bifurcation in IL-7Ra+ regions, and LFA-1 fluctuates positively in the pDCdirection at the bifurcation. These expectancies are validated by the presence of B/pDCprogenitors in the IL-7Ra+ fraction and preferential expression of LFA-1 in pDC-biased progenitors with a niche-like culture system.Weprovide a model of fluctuation-based differentiation, which reconciles continuous and discrete models and is applicable to other developmental systems.
本文/Department of Hematology and Oncology, Mie University Graduate School of Medicine, Tsu 514-8507, Japan
22p
Persistent ID (NDL): info:ndljp/pid/13024219
https://dl.ndl.go.jp/pid/13024219
Collection: 障害者向け資料
Collection (Materials For Handicapped People:1): テキストデータ
Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文
https://dl.ndl.go.jp/collections/A00014
Acquisition Basis: 博士論文（自動収集）
Date Accepted (W3CDTF): 2023-10-11T15:41:06+09:00
Format (IMT): application/pdf
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Periodical Title (URI): http://hdl.handle.net/10076/00021136
https://mie-u.repo.nii.ac.jp/records/15520
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