Iroquois homeobox 3 regulates odontoblast proliferation and differentiation mediated by Wnt5a expression

• Material Type: 博士論文
• Title: Iroquois homeobox 3 regulates odontoblast proliferation and differentiation mediated by Wnt5a expression
• Author/Editor: Narwidina, Anrizandy; Miyazaki, Aya; Iwata, Kokoro; Kurogoushi, Rika; Sugimoto, Asuna; Kudo, Yasusei; Kawarabayashi, Keita; Yamakawa, Yoshihito; Akazawa, Yuki; Kitamura, Takamasa; Nakagawa, Hiroshi; Yamaguchi-Ueda, Kimiko; Hasegawa, Tomokazu; Yoshizaki, Keigo; Fukumoto, Satoshi; Yamamoto, Akihito; Ishimaru, Naozumi; Iwasaki, Tomonori; Iwamoto, Tsutomu
• Author Heading: Narwidina, Anrizandy; Miyazaki, Aya; Iwata, Kokoro; Kurogoushi, Rika; Sugimoto, Asuna; Kudo, Yasusei; Kawarabayashi, Keita; Yamakawa, Yoshihito; Akazawa, Yuki; Kitamura, Takamasa; Nakagawa, Hiroshi; Yamaguchi-Ueda, Kimiko; Hasegawa, Tomokazu; Yoshizaki, Keigo; Fukumoto, Satoshi; Yamamoto, Akihito; Ishimaru, Naozumi; Iwasaki, Tomonori; Iwamoto, Tsutomu
• Publication, Distribution, etc.: Elsevier
• Publication Date: 2023-02-04
• Publication Date (W3CDTF): 2023-02-04
• Alternative Title: ホメオボックス型転写因子Irx3はWnt5aの発現に関与し象牙芽細胞の増殖と分化に調整する
• Degree grantor/type: 徳島大学
• Date Granted: 2023-09-20
• Date Granted (W3CDTF): 2023-09-20
• Dissertation Number: 甲第3748号
• Degree Type: 博士（歯学）
• Text Language Code: eng
• Subject Heading: Iroquois homeobox genes; Irx3; Odontoblasts; Cell proliferation; Cell differentiation; Wnt5a
• Target Audience: 一般
• Note (General): Iroquois homeobox (Irx) genes are TALE-class homeobox genes that are evolutionarily conserved across species and have multiple critical cellular functions in fundamental tissue development processes. Previous studies have shown that Irxs genes are expressed during tooth development. However, the precise roles of genes in teeth remain unclear. Here, we demonstrated for the first time that Irx3 is an essential molecule for the proliferation and differentiation of odontoblasts. Using cDNA synthesized from postnatal day 1 (P1) tooth germs, we examined the expression of all Irx genes (Irx1-Irx6) by RT-PCR and found that all genes except Irx4 were expressed in the tooth tissue. Irx1-Irx3 a were expressed in the dental epithelial cell line M3H1 cells, while Irx3 and Irx5 were expressed in the dental mesenchymal cell line mDP cells. Only Irx3 was expressed in both undifferentiated cell lines. Immunostaining also revealed the presence of IRX3 in the dental epithelial cells and mesenchymal condensation. Inhibition of endogenous Irx3 by siRNA blocks the proliferation and differentiation of mDP cells. Wnt3a, Wnt5a, and Bmp4 are factors involved in odontoblast differentiation and were highly expressed in mDP cells by quantitative PCR analysis. Interestingly, the expression of Wnt5a (but not Wnt3a or Bmp4) was suppressed by Irx3 siRNA. These results suggest that Irx3 plays an essential role in part through the regulation of Wnt5a expression during odontoblast proliferation and differentiation.
• Persistent ID (NDL): info:ndljp/pid/13119246; https://dl.ndl.go.jp/pid/13119246
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• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2023-12-05T21:40:56+09:00
• Format (IMT): application/pdf
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• Periodical Title (URI): http://repo.lib.tokushima-u.ac.jp/118052
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