Involvement of α-klotho in growth hormone (GH) signaling

• Material Type: 博士論文
• Title: Involvement of α-klotho in growth hormone (GH) signaling
• Author/Editor: Koike, Megumi; Sato, Tetsuhiko; Shiozaki, Yuji; Komiya, Aoi; Miura, Mizuki; Higashi, Ayami; Ishikawa, Akane; Takayanagi, Kaori; Uga, Minori; Miyamoto, Ken-ichi; Segawa, Hiroko
• Author Heading: Koike, Megumi; Sato, Tetsuhiko; Shiozaki, Yuji; Komiya, Aoi; Miura, Mizuki; Higashi, Ayami; Ishikawa, Akane; Takayanagi, Kaori; Uga, Minori; Miyamoto, Ken-ichi; Segawa, Hiroko
• Publication, Distribution, etc.: Society for Free Radical Research Japan
• Publication Date: 2024; 74 3
• Publication Date (W3CDTF): 2024
• Alternative Title: 成長ホルモンシグナルにおけるα-klothoの関与
• Degree grantor/type: 徳島大学
• Date Granted: 2024-02-22
• Date Granted (W3CDTF): 2024-02-22
• Dissertation Number: 甲第3775号
• Degree Type: 博士（栄養学）
• Text Language Code: eng
• Subject Heading: growth hormone; α-klotho; kidney; CKD; GH-resistant
• Target Audience: 一般
• Note (General): Growth hormone (GH) exerts multiple effects on different organs directly or via its main mediator, insulin-like growth factor1 (IGF1). In this study, we focused on the novel relationship between GH action and the antiaging hormone α-klotho. Immunofluorescent staining of α-klotho was observed in the renal distal tubules and pituitary glands of somatostatin- and GH-positive cells in wild-type (WT) mice. Treatment of 4-week-old WT mice with GH increased IGF1 mRNA expression in the pituitary gland, liver, heart, kidney, and bone but increased α-klotho mRNA expression only in the pituitary gland, kidney, and bone. Increased α-klotho protein levels were observed in the kidney but not in the pituitary gland. No induction of α-klotho RNA expression by GH was observed in juvenile mice with kidney disease, indicating GH resistance. Furthermore, GH and α-klotho supplementation in HEK293 cells transfected with GHR increased Janus kinase 2 mRNA (a GH downstream signal) expression compared to supple­mentation with GH alone. In conclusion, we suggest that 1) the kidney is the main source of secreted α-klotho, which is detected in blood by the downstream action of GH, 2) α-klotho induction by GH is resistant in kidney disease, and 3) α-klotho might be an enhanced regulator of GH signaling.
• Persistent ID (NDL): info:ndljp/pid/13721951; https://dl.ndl.go.jp/pid/13721951
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2024-07-05T22:06:55+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
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• Periodical Title (URI): http://repo.lib.tokushima-u.ac.jp/119161
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp