Characterization of Outer Membrane Vesicles Produced by Vibrio vulnificus

• Material Type: 博士論文
• Title: Characterization of Outer Membrane Vesicles Produced by Vibrio vulnificus
• Author/Editor: Higashiyama, Ryo; Kanda, Yuna; Shimohata, Takaaki; Ishida, Kai; Fukushima, Shiho; Yamazaki, Kohei; Uebanso, Takashi; Mawatari, Kazuaki; Kashimoto, Takashige; Takahashi, Akira
• Author Heading: Higashiyama, Ryo; Kanda, Yuna; Shimohata, Takaaki; Ishida, Kai; Fukushima, Shiho; Yamazaki, Kohei; Uebanso, Takashi; Mawatari, Kazuaki; Kashimoto, Takashige; Takahashi, Akira
• Publication, Distribution, etc.: Tokushima University Faculty of Medicine
• Publication Date: 2024-02; 71 1-2
• Publication Date (W3CDTF): 2024-02
• Alternative Title: Vibrio vulnificusから産生される細胞外小胞の特徴について; Characterization of OMVs by V. vulnificus
• Degree grantor/type: 徳島大学
• Date Granted: 2024-02-22
• Date Granted (W3CDTF): 2024-02-22
• Dissertation Number: 甲第3774号
• Degree Type: 博士（栄養学）
• Text Language Code: eng
• Subject Heading: Infection; Outer Membrane Vesicle; Pathogenicity; Serine Protease; Vibrio Vulnificus
• Target Audience: 一般
• Note (General): Vibrio vulnificus (V. vulnificus) is a halophilic gram-negative bacterium that inhabits coastal warm water and induce severe diseases such as primary septicemia. To investigate the mechanisms of rapid bacterial translocation on intestinal infection, we focused on outer membrane vesicles (OMVs), which are extracellular vesicles produced by Gram-negative bacteria and deliver virulence factors. However, there are very few studies on the pathogenicity or contents of V. vulnificus OMVs (Vv-OMVs). In this study, we investigated the effects of Vv-OMVs on host cells. Epithelial cells INT407 were stimulated with purified OMVs and morphological alterations and levels of lactate dehydrogenase (LDH) release were observed. In cells treated with OMVs, cell detachment without LDH release was observed, which exhibited different characteristics from cytotoxic cell detachment observed in V. vulnificus infection. Interestingly, OMVs from a Vibrio Vulnificus Hemolysin (VVH) and Multifunctional-autoprocessing repeats-in -toxin (MARTX) double-deletion mutant strain also caused cell detachment without LDH release. Our results suggested that the proteolytic function of a serine protease contained in Vv-OMVs may contribute to pathogenicity of V. vulnificus by assisting bacterial translocation. This study reveals a new pathogenic mechanism during V. vulnificus infections.
• Persistent ID (NDL): info:ndljp/pid/13721956; https://dl.ndl.go.jp/pid/13721956
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2024-07-05T22:06:55+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://repo.lib.tokushima-u.ac.jp/119259
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp