Culture of organoids with vestibular cell-derived factors promotes differentiation of embryonic stem cells into inner ear vestibular hair cells

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Culture of organoids with vestibular cell-derived factors promotes differentiation of embryonic stem cells into inner ear vestibular hair cells

Persistent ID (NDL): info:ndljp/pid/13805828

Material type: 博士論文

Author: Osaki, Daisukeほか

Publisher: Elsevier

Publication date: 2023-02

Material Format: Digital

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Name of awarding university/degree: 奈良県立医科大学,博士（医学）

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Note (General)：: type:ThesisVestibular hair cells (V-HCs) residing in the inner ear have important roles related to balance. Although differentiation of pluripotent st...

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Material Type: 博士論文
Title: Culture of organoids with vestibular cell-derived factors promotes differentiation of embryonic stem cells into inner ear vestibular hair cells
Author/Editor: Osaki, Daisuke
Ouji, Yukiteru
Sakagami, Masaharu
Kitamura, Tomotaka
Misu, Masayasu
Kitahara, Tadashi
Yoshikawa, Masahide
Author Heading: Osaki, Daisuke
Ouji, Yukiteru
Sakagami, Masaharu
Kitamura, Tomotaka
Misu, Masayasu
Kitahara, Tadashi
Yoshikawa, Masahide
Publication, Distribution, etc.: Elsevier
Publication Date: 2023-02
135 2
Publication Date (W3CDTF): 2023-02
Alternative Title: 内耳前庭細胞由来因子とオルガノイド培養を組み合わせたES細胞から内耳前庭有毛細胞への分化誘導
Degree grantor/type: 奈良県立医科大学
Date Granted: 2023-03-15
Date Granted (W3CDTF): 2023-03-15
Dissertation Number: 甲第877号
Degree Type: 博士（医学）
Text Language Code: eng
Subject Heading: Conditioned medium
Differentiation
Embryonic stem cells
Hair cells
Organoid
Vestibular
Target Audience: 一般
Note (General): type:Thesis
Vestibular hair cells (V-HCs) residing in the inner ear have important roles related to balance. Although differentiation of pluripotent stem cells into HCs has been shown, an effective method has yet to be established. We previously reported that use of vestibular cell-derived conditioned medium (V-CM) was helpful to induce embryonic stem (ES) cells to differentiate into V-HC-like cells in two-dimensional (2D) cultures of ES-derived embryoid bodies (EBs). In the present report, V-CM was used with three-dimensional (3D) cultures of EBs, which resulted in augmented expression of V-HC-related markers (Math1, Myosin6, Brn3c, Dnah5), but not of the cochlear HC-related marker Lmod3. Gene expression analyses of both 2D and 3D EBs cultured for two weeks revealed a greater level of augmented induction of HC-related markers in the 3D-cultured EBs. These results indicate that a 3D culture in combination with use of V-CM is an effective method for producing V-HCs.
博士（医学）・甲第877号・令和5年3月15日
identifier:Journal of bioscience and bioengineering Vol.135 No.2 p.143-150 (2023 Feb)
identifier:13891723
identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/4109
identifier:Journal of bioscience and bioengineering, 135(2): 143-150
Persistent ID (NDL): info:ndljp/pid/13805828
https://dl.ndl.go.jp/pid/13805828
Collection: 障害者向け資料
Collection (Materials For Handicapped People:1): テキストデータ
Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文
https://dl.ndl.go.jp/collections/A00014
Acquisition Basis: 博士論文（自動収集）
Date Accepted (W3CDTF): 2024-11-12T17:30:04+09:00
Format (IMT): application/pdf
Access Restrictions: 国立国会図書館内限定公開
Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
Availability of remote photoduplication service: 可
Periodical Title (URI): http://hdl.handle.net/10564/4109
Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション
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