低出力超音波パルスはBMP9により誘導されたラット脱分化脂肪細胞の骨芽細胞様分化を促進する

• Material Type: 博士論文
• Title: 低出力超音波パルスはBMP9により誘導されたラット脱分化脂肪細胞の骨芽細胞様分化を促進する
• Author/Editor: 瀬戸口, 史晃
• Author Heading: 瀬戸口, 史晃
• Publication, Distribution, etc.: 鹿児島大学
• Publication Date: 2023-09-21
• Publication Date (W3CDTF): 2023-09-21
• Alternative Title: Low-Intensity Pulsed Ultrasound Promotes BMP9 Induced Osteoblastic Differentiation in Rat Dedifferentiated Fat Cells
• Degree Grantor: 鹿児島大学
• Date Granted: 2023-09-21
• Date Granted (W3CDTF): 2023-09-21
• Dissertation Number: 甲総研第713号
• Degree Type: 博士(歯学)
• Text Language Code: eng
• Subject Heading: Dedifferentiated fat cells; Bone morphogenetic protein 9; Low-intensity pulsed ultrasound; Prostaglandins
• Target Audience: 一般
• Note (General): 出版タイプ： VoR; 博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨; Dedifferentiated fat cells (DFATs) isolated from mature adipocytes have a multilineage differentiation capacity similar to mesenchymal stem cells and are considered as promising source of cells for tissue engineering. Bone morphogenetic protein 9 (BMP9) and low-intensity pulsed ultrasound (LIPUS) have been reported to stimulate bone formation both in vitro and in vivo. However, the combined effect of BMP9 and LIPUS on osteoblastic differentiation of DFATs has not been studied. After preparing DFATs from mature adipose tissue from rats, DFATs were treated with different doses of BMP9 and/or LIPUS. The effects on osteoblastic differentiation were assessed by changes in alkaline phosphatase (ALP) activity, mineralization/calcium deposition, and expression of bone related genes; Runx2, osterix, osteopontin. No significant differences for ALP activity, mineralization deposition, as well as expression for bone related genes were observed by LIPUS treatment alone while treatment with BMP9 induced osteoblastic differentiation of DFATs in a dose dependent manner. Further, co-treatment with BMP9 and LIPUS significantly increased osteoblastic differentiation of DFATs compared to those treated with BMP9 alone. In addition, upregulation for BMP9-receptor genes was observed by LIPUS treatment. Indomethacin, an inhibitor of prostaglandin synthesis, significantly inhibited the synergistic effect of BMP9 and LIPUS co-stimulation on osteoblastic differentiation of DFATs. LIPUS promotes BMP9 induced osteoblastic differentiation of DFATs in vitro and prostaglandins may be involved in this mechanism.; Fumiaki Setoguchi, Kotaro Sena, Kazuyuki NoguchiLow-Intensity Pulsed Ultrasound Promotes BMP9 Induced Osteoblastic Differentiation in Rat Dedifferentiated Fat CellsInternational Journal of Stem Cells 2023;16:406-414https://doi.org/10.15283/ijsc23027
• Persistent ID (NDL): info:ndljp/pid/14436682; https://dl.ndl.go.jp/pid/14436682
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2025-07-04T22:06:23+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://hdl.handle.net/10232/0002000018
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp