Immunohistochemical localization of mesenchymal stem cells in ossified human spinal ligaments 4

• Material Type: 博士論文
• Title: Immunohistochemical localization of mesenchymal stem cells in ossified human spinal ligaments
• Volume: 436 4
• Author/Editor: Chin, Shunfu; Furukawa, Ken-Ichi; Ono, Atsushi; Asari, Toru; Harada, Yoshifumi; Wada, Kanichiro; Tanaka, Toshihiro; Inaba, Wataru; Mizukami, Hiroki; Motomura, Shigeru; Yagihashi, Soroku; Ishibashi, Yasuyuki
• Author Heading: Chin, Shunfu; Furukawa, Ken-Ichi; Ono, Atsushi; Asari, Toru; Harada, Yoshifumi; Wada, Kanichiro; Tanaka, Toshihiro; Inaba, Wataru; Mizukami, Hiroki; Motomura, Shigeru; Yagihashi, Soroku; Ishibashi, Yasuyuki
• Publication, Distribution, etc.: Elsevier
• Publication Date: 2013-06
• Publication Date (W3CDTF): 2013-06
• Periodical title: Biochemical and biophysical research communications
• Degree grantor/type: 弘前大学
• Date Granted: 2013-09-30
• Date Granted (W3CDTF): 2013-09-30
• Dissertation Number: 甲第1811号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 甲第1811号
• Text Language Code: eng
• Subject Heading: Spinal ligament ossification; Mesenchymal stem cells; Immunohistochemistry; Localization; Neovascularization; Chondrocytes; 外科系臨床医学
• NDC: 494.66
• Target Audience: 一般
• Note (General): type:Thesis; Mesenchymal stem cells (MSCs) have been isolated from various tissues and used for elucidating the pathogenesis of numerous diseases. In our previous in vitro study, we showed the existence of MSCs in human spinal ligaments and hypothesized that these MSCs contributed to the pathogenesis of ossification of spinal ligaments. The purpose of this study was to use immunohistochemical techniques to analyze the localization of MSCs in ossified human spinal ligaments in situ. Ossified (OLF) or non-ossified ligamentum flavum (non-OLF) samples from the thoracic vertebra were obtained from patients who had undergone posterior spinal surgery. Serial sections were prepared from paraffin-embedded samples, and double immunofluorescence staining was performed using antibodies against markers for MSCs (CD73, CD90 and CD105), endothelial cells (CD31), pericytes (α-smooth muscle actin), and chondrocytes (S100). Immunolocalization of MSCs was observed in the perivascular area and collagenous matrix in spinal ligaments. Markers for MSCs and pericytes were co-expressed in the perivascular area. Compared with non-OLF, OLF had a large amount of neovascularization in the fragmented ligament matrix, and a high accumulation of MSCs around blood vessels. The prevalence of MSCs in OLF within collagenous matrix was significantly higher than that in non-OLF. Chondrocytes near the ossification front in OLF also presented expression of MSC markers. MSCs may contribute to the ectopic ossification process of OLF through endochondral ossification.; identifier:Biochemical and biophysical research communications, 436(4), 2013, p.698-704
• DOI: info:doi/10.1016/j.bbrc.2013.06.019; https://doi.org/info:doi/10.1016/j.bbrc.2013.06.019
• Persistent ID (NDL): info:ndljp/pid/8948622; https://dl.ndl.go.jp/pid/8948622
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Available (W3CDTF): 2015-03-03
• Date Accepted (W3CDTF): 2015-02-03T05:25:05+09:00
• Date Created (W3CDTF): 2018-03-13
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://hdl.handle.net/10129/5113
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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