Matrix-assisted laser desorption/ionization imaging mass spectrometry revealed traces of dental problem associated with dental structure

• Material Type: 博士論文
• ISSN: 1618-2642
• Title: Matrix-assisted laser desorption/ionization imaging mass spectrometry revealed traces of dental problem associated with dental structure
• Author/Editor: Hirano, Hirokazu
• Author Heading: Hirano, Hirokazu
• Publication, Distribution, etc.: Springer Berlin Heidelberg
• Publication Date: 2013
• Publication Date (W3CDTF): 2013
• Alternative Title: マトリックス支援レーザー脱離イオン化質量分析イメージング法は歯の構造と関連する歯科疾患の痕跡を明らかにした
• Degree grantor/type: 浜松医科大学
• Date Granted: 2013-09-20
• Date Granted (W3CDTF): 2013-09-20
• Dissertation Number: 甲第661号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 甲第661号
• Text Language Code: eng
• Subject Heading: Imaging Mass Spectrometry (IMS); Matrix Assisted Laser Ionization/Desorption (MALDI); dental structure; periodontal disease; lysophosphatidylcholine (LPC)
• Target Audience: 一般
• Note (General): doctoral; 医学系研究科; Periodontal disease is a serious dental problem because it doesn’t heal naturally and leads to tooth loss. In periodontal disease, inflammation at periodontal tissue is thought as predominant, and its effect against tooth itself remains unclear. In this study, we applied matrix assisted laser desorption/ionization (MALDI) Imaging Mass Spectrometry (IMS) to teeth for the first time. By comparing anatomical structure of tooth affected with periodontal disease with normal ones, we analyzed traces of the disease on tooth. We found signals characteristic of enamel, dentin, and dental pulp, respectively, in mass spectra obtained from normal teeth. Ion images reconstructed using these signals showed anatomical structures of the tooth clearly. Next, we performed IMS upon teeth of periodontal disease. Overall characteristic of the mass spectrum appeared similar to normal ones. However, ion images reconstructed using signals from the tooth of periodontal disease revealed loss of periodontal ligament visualized together with dental pulp in normal teeth. Moreover, ion image clearly depicted an accumulation of signal at m/z 496.3 at root surface. Such an accumulation that cannot be examined only from mass spectrum was revealed by utilization of IMS. Recent studies about inflammation revealed that the signal at m/z 496.3 reflects lyso-phosphatidylcholine (LPC). Infiltration of the signal is statistically significant and its intensity profile exhibited the influence has reached deeply into the tooth. This suggests that influence of periodontal disease is not only inflammation of periodontal tissue but also infiltration of LPC to root surface, and therefore, anti-inflammatory treatment is required besides conventional treatments. (250 words)
• DOI: info:doi/10.1007/s00216-013-7075-y; https://doi.org/info:doi/10.1007/s00216-013-7075-y
• Persistent ID (NDL): info:ndljp/pid/8952111; https://dl.ndl.go.jp/pid/8952111
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Available (W3CDTF): 2015-03-03
• Date Accepted (W3CDTF): 2015-02-03T05:25:05+09:00
• Date Created (W3CDTF): 2018-08-27
• Format (IMT): application/pdf; text/html
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://hdl.handle.net/10271/2775; https://hama-med.repo.nii.ac.jp/records/2648
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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