Yb Integrates piRNA Intermediates and Processing Factors into Perinuclear Bodies to Enhance piRISC Assembly 1

• Material Type: 博士論文
• ISSN: 2211-1247
• Title: Yb Integrates piRNA Intermediates and Processing Factors into Perinuclear Bodies to Enhance piRISC Assembly
• Volume: 8 1
• Author/Editor: Murota, Yukiko; Ishizu, Hirotsugu; Nakagawa, Shinichi; Iwasaki, Yuka W.; Shibata, Shinsuke; Kamatani, Miharu K.; Saito, Kuniaki; Okano, Hideyuki; Shiomi, Haruhiko; Siomi, Mikiko C.
• Author Heading: Murota, Yukiko; Ishizu, Hirotsugu; Nakagawa, Shinichi; Iwasaki, Yuka W.; Shibata, Shinsuke; Kamatani, Miharu K.; Saito, Kuniaki; Okano, Hideyuki; Shiomi, Haruhiko; Siomi, Mikiko C.
• Publication, Distribution, etc.: Elsevier
• Publication Date: 2014-06-19
• Publication Date (W3CDTF): 2014-06-19
• Alternative Title: YbはpiRNA前駆体とpiRNA生合成因子を核周辺部位に形成される顆粒体に局在させることによってpiRISC形成を促進する
• Periodical title: Cell Reports
• Degree grantor/type: 徳島大学
• Date Granted: 2014-10-23
• Date Granted (W3CDTF): 2014-10-23
• Dissertation Number: 甲第2779号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 甲第2779号
• Text Language Code: eng
• Subject Heading: piRNA; Piwi; flamenco; Yb body; Drosophila
• Target Audience: 一般
• Note (General): PIWI-interacting RNAs (piRNAs) direct Piwi to repress transposons and maintain genome integrity in Drosophila ovarian somatic cells. piRNA maturation and association with Piwi occur at perinuclear Yb bodies, the centers of piRNA biogenesis. Here, we show that piRNA intermediates arising from the piRNA cluster flamenco (flam) localize to perinuclear foci adjacent to Yb bodies, termed Flam bodies. RNAi-based screening of piRNA factors revealed that Flam body formation depends on Yb, the core component of Yb bodies, while Piwi and another Yb body component, Armitage, are dispensable for formation. Abolishing the RNA-binding activity of Yb disrupts both Flam bodies and Yb bodies. Yb directly binds flam, but not transcripts from neighboring protein-coding genes. Thus, Yb integrates piRNA intermediates and piRNA processing factors selectively into Flam bodies and Yb bodies, respectively. We suggest that Yb is a key upstream factor in the cytoplasmic phase of the piRNA pathway in ovarian somatic cells.
• DOI: info:doi/10.1016/j.celrep.2014.05.043; https://doi.org/info:doi/10.1016/j.celrep.2014.05.043
• Persistent ID (NDL): info:ndljp/pid/8952372; https://dl.ndl.go.jp/pid/8952372
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Available (W3CDTF): 2015-03-03
• Date Accepted (W3CDTF): 2015-02-03T05:25:05+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://repo.lib.tokushima-u.ac.jp/106315
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp