Differential expression of hepatic apurinic/apyrimidinic endonuclease 1, a DNA repair enzyme, in chronic hepatitis

• Material Type: 博士論文
• Title: Differential expression of hepatic apurinic/apyrimidinic endonuclease 1, a DNA repair enzyme, in chronic hepatitis
• Author/Editor: Sumiyoshi, Shinichi
• Author Heading: Sumiyoshi, Shinichi
• Publication, Distribution, etc.: Baishideng
• Publication Date: 2013
• Publication Date (W3CDTF): 2013
• Alternative Title: 慢性肝炎における肝臓のDNA修復酵素apurinic/apyrimidinic endonuclease 1の特異な発現について
• Degree grantor/type: 浜松医科大学
• Date Granted: 2014-01-17
• Date Granted (W3CDTF): 2014-01-17
• Dissertation Number: 乙第510号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 乙第510号
• Text Language Code: eng
• Subject Heading: Apurinic/apyrimidinic endonuclease 1; 8-hydroxydeoxyguanosine; Oxidative stress; Viral hepatitis
• Target Audience: 一般
• Note (General): doctoral; 医学系研究科; AIM: To determine hepatic expression of apurinic/apyrimidinic endonuclease 1 (APE-1) and 8-hydroxydeoxyguanosine (8-OHdG) in patients with chronic hepatitis B and C.METHODS: Liver biopsies were obtained from 27 patients with chronic hepatitis B virus (HBV), 30 with chronic hepatitis C virus (HCV), 6 with autoimmune hepatitis (AIH), and 6 with primary biliary cirrhosis (PBC). Normal liver tissue was obtained from surgical resection specimens of four patients. Hepatic APE-1 protein and mRNA expression were assayed by Western blot and by real-time polymerase chain reaction, respectively. Hepatocellular APE-1 and 8-OHdG expression were determined by immunohistochemistry.RESULTS: The staining intensity of hepatocellular nuclear APE-1 was lower in the HBV group than in the other groups (P<0.05). Hepatic APE-1 protein levels were reduced in the HBV group relative to the other groups. Hepatic APE-1 mRNA levels were also lower in the HBV group. The proportion of hepatocytes with 8-OHdG-positive nuclei was increased in the HCV, AIH and PBC groups (P<0.05), but not in the HBV group. Hepatocellular nuclear APE-1 levels were positively correlated with hepatocellular 8-OHdG levels in both the HBV and HCV groups (HBV, r=0.34, P<0.05; HCV, r=0.54, P<0.01).CONCLUSION: An imbalance between oxidative DNA damage and APE-1 expression may contribute to hepatocarcinogenesis in chronic viral hepatitis.
• DOI: info:doi/10.4254/wjh.v5.i4.206; https://doi.org/info:doi/10.4254/wjh.v5.i4.206
• Persistent ID (NDL): info:ndljp/pid/8952980; https://dl.ndl.go.jp/pid/8952980
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Available (W3CDTF): 2015-03-03
• Date Accepted (W3CDTF): 2015-02-03T05:25:05+09:00
• Date Created (W3CDTF): 2018-08-27
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://hdl.handle.net/10271/2779; https://hama-med.repo.nii.ac.jp/records/2660
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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