Azelnidipine inhibits cultured rat aortic smooth muscle cell death induced by cyclic mechanical stretch. 7

• Material Type: 博士論文
• ISSN: 1932-6203
• Title: Azelnidipine inhibits cultured rat aortic smooth muscle cell death induced by cyclic mechanical stretch.
• Volume: 9 7
• Author/Editor: Zhao, Jing; Ozawa, Kentaro; Kyotani, Yoji; Nagayama, Kosuke; Ito, Satoyasu; Komatsubara, Akira T.; Tsuji, Yuichi; Yoshizumi, Masanori
• Author Heading: Zhao, Jing; Ozawa, Kentaro; Kyotani, Yoji; Nagayama, Kosuke; Ito, Satoyasu; Komatsubara, Akira T.; Tsuji, Yuichi; Yoshizumi, Masanori
• Publication, Distribution, etc.: Public Library of Science
• Publication Date: 2014-07-17; 9 7
• Publication Date (W3CDTF): 2014-07-17
• Alternative Title: アゼルニジピンは周期性伸展負荷による培養ラット大動脈平滑筋細胞死を抑制する
• Periodical title: PLoS ONE
• Degree grantor/type: 奈良県立医科大学
• Date Granted: 2014-12-03
• Date Granted (W3CDTF): 2014-12-03
• Dissertation Number: 乙第1344号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 乙第1344号
• Text Language Code: eng
• Target Audience: 一般
• Note (General): type:Thesis; Acute aortic dissection is the most common life-threatening vascular disease, with sudden onset of severe pain and a high fatality rate. Clarifying the detailed mechanism for aortic dissection is of great significance for establishing effective pharmacotherapy for this high mortality disease. In the present study, we evaluated the influence of biomechanical stretch, which mimics an acute rise in blood pressure using an experimental apparatus of stretching loads in vitro, on rat aortic smooth muscle cell (RASMC) death. Then, we examined the effects of azelnidipine and mitogen-activated protein kinase inhibitors on mechanical stretch-induced RASMC death. The major findings of the present study are as follows: (1) cyclic mechanical stretch on RASMC caused cell death in a time-dependent manner up to 4 h; (2) cyclic mechanical stretch on RASMC induced c-Jun N-terminal kinase (JNK) and p38 activation with peaks at 10 min; (3) azelnidipine inhibited RASMC death in a concentration-dependent manner as well as inhibited JNK and p38 activation by mechanical stretch; and (4) SP600125 (a JNK inhibitor) and SB203580 (a p38 inhibitor) protected against stretch-induced RASMC death; (5) Antioxidants, diphenylene iodonium and tempol failed to inhibit stretch-induced RASMC death. On the basis of the above findings, we propose a possible mechanism where an acute rise in blood pressure increases biomechanical stress on the arterial walls, which induces RASMC death, and thus, may lead to aortic dissection. Azelnidipine may be used as a pharmacotherapeutic agent for prevention of aortic dissection independent of its blood pressure lowering effect.; 博士（医学）・乙第1344号・平成26年12月3日; © 2014 Zhao et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.; identifier:PLoS ONE Vol.9 No.7 Article No.e102813 (2014 Jul); identifier:19326203; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/2748; identifier:PLoS ONE, 9(7): Article No.e102813
• DOI: info:doi/10.1371/journal.pone.0102813; https://doi.org/info:doi/10.1371/journal.pone.0102813
• Persistent ID (NDL): info:ndljp/pid/8953110; https://dl.ndl.go.jp/pid/8953110
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Available (W3CDTF): 2015-03-03
• Date Accepted (W3CDTF): 2015-02-03T05:25:05+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://hdl.handle.net/10564/2748
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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