Direct isolation of myofibroblasts and fibroblasts from bleomycin-injured lungs reveals their functional similarities and differences

• Material Type: 博士論文
• Title: Direct isolation of myofibroblasts and fibroblasts from bleomycin-injured lungs reveals their functional similarities and differences
• Author/Editor: Akamatsu, Taisuke
• Author Heading: Akamatsu, Taisuke
• Publication, Distribution, etc.: BioMed Central
• Publication Date: 2013-08-08
• Publication Date (W3CDTF): 2013-08-08
• Alternative Title: ブレオマイシン傷害肺からの筋線維芽細胞と線維芽細胞の直接単離は両細胞の機能的な類似性や差異を明らかにする
• Degree grantor/type: 浜松医科大学
• Date Granted: 2014-03-17
• Date Granted (W3CDTF): 2014-03-17
• Dissertation Number: 甲第674号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 甲第674号
• Text Language Code: eng
• Subject Heading: α-smooth muscle actin; Chemokine; Collagen; Fibroblasts; Myofibroblasts
• Target Audience: 一般
• Note (General): doctoral; 医学系研究科; Background: Myofibroblasts play a crucial role in tissue repair. The functional similarities and differences between myofibroblasts and fibroblasts are not fully understood because they have not been separately isolated from a living body. The purpose of this study was to establish a method for the direct isolation of myofibroblasts and fibroblasts from injured lungs by using fluorescence-activated cell sorting and to compare their functions.Results: We demonstrated that lineage-specific cell surface markers (lin), such as CD31, CD45, CD146, EpCAM (CD326), TER119, and Lyve-1 were not expressed in myofibroblasts or fibroblasts. Fibroblasts of bleomycin-injured lungs and saline-treated lungs were shown to be enriched in linneg Sca-1high, and myofibroblasts of bleomycininjured lungs were shown to be enriched in linneg Sca-1low CD49ehigh. Results from in-vitro proliferation assays indicated in-vitro proliferation of fibroblasts but not myofibroblasts of bleomycin-injured lungs and of fibroblasts of saline-treated lungs. However, fibroblasts and myofibroblasts might have a low proliferative capacity in vivo. Analysis of genes for collagen and collagen synthesis enzymes by qRT-PCR showed that the expression levels of about half of the genes were significantly higher in fibroblasts and myofibroblasts of bleomycin-injured lungs than in fibroblasts of saline-treated lungs. By contrast, the expression levels of 8 of 11 chemokine genes of myofibroblasts were significantly lower than those of fibroblasts.Conclusions: This is the first study showing a direct isolation method of myofibroblasts and fibroblasts from injured lungs. We demonstrated functional similarities and differences between myofibroblasts and fibroblasts in terms of both their proliferative capacity and the expression levels of genes for collagen, collagen synthesis enzymes, and chemokines. Thus, this direct isolation method has great potential for obtaining useful information from myofibroblasts and fibroblasts.
• DOI: info:doi/10.1186/1755-1536-6-15; https://doi.org/info:doi/10.1186/1755-1536-6-15
• Persistent ID (NDL): info:ndljp/pid/8953194; https://dl.ndl.go.jp/pid/8953194
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Available (W3CDTF): 2015-03-03
• Date Accepted (W3CDTF): 2015-02-03T05:25:05+09:00
• Date Created (W3CDTF): 2018-08-27
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://hdl.handle.net/10271/2784; https://hama-med.repo.nii.ac.jp/records/2652
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

Table of Contents

2018-10-04 再収集

2023-03-02 再収集

2023-10-11 再収集

2023-12-09 再収集