Immunohistochemical localization of an isoform of TRK-fused gene-like protein in the rat retina.

• Material Type: 博士論文
• ISSN: 0044-5991
• Title: Immunohistochemical localization of an isoform of TRK-fused gene-like protein in the rat retina.
• Volume: 47 2
• Author/Editor: MASUDA, Chiaki
• Author Heading: MASUDA, Chiaki
• Publication, Distribution, etc.: Japan Society of Histochemistry and Cytochemistry
• Publication Date: 2014-04-25
• Publication Date (W3CDTF): 2014-04-25
• Periodical title: Acta Histochemica et Cytochemica
• Degree grantor/type: 滋賀医科大学
• Date Granted: 2014-09-10
• Date Granted (W3CDTF): 2014-09-10
• Dissertation Number: 甲第712号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 甲第712号
• Text Language Code: eng
• Subject Heading: amacrine cells; antibodies; horizontal cells; immunohistochemistry; retina
• Target Audience: 一般
• Note (General): The TRK-fused gene (TFG) was originally identified in chromosome translocation events, creating a pair of oncogenes in some cancers, and was recently demonstrated as the causal gene of hereditary motor and sensory neuropathy with proximal dominant involvement. Recently, we cloned an alternative splicing variant of Tfg from a cDNA library of the rat retina, tentatively naming it retinal Tfg (rTfg). Although the common form of Tfg is ubiquitously expressed in most rat tissues, rTfg expression is localized to the central nervous system. In this study, we produced an antibody against an rTFG-specific amino acid sequence and used it to examine the localization of rTFG-like protein in the rat retina by immunohistochemistry and Western blots. Western blot analysis showed that the antibody detected a single band of 24 kDa in the rat retina. When we examined rTFG recombinant protein, the antibody detected two bands of about 42 kDa and 24 kDa. The results suggest that the 24 kDa rTFG-like protein is a fragment of rTFG. In our immunohistochemical studies of the rat retina, rTFG-like immunoreactivity was observed in all calbindin D-28K-positive horizontal cells and in some syntaxin 1-positive amacrine cells (ACs). In addition, the rTFG-like immunopositive ACs were actually glycine transporter 1-positive glycinergic or glutamate decarboxylase-positive GABAergic ACs. Our findings indicate that this novel 24 kDa rTFG-like protein may play a specific role in retinal inhibitory; Thesis or Dissertation; 平成26年度; 10.1267/ahc.14018; J-STAGE
• DOI: info:doi/10.1267/ahc.14018; https://doi.org/info:doi/10.1267/ahc.14018
• Persistent ID (NDL): info:ndljp/pid/8953483; https://dl.ndl.go.jp/pid/8953483
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Available (W3CDTF): 2015-03-03
• Date Accepted (W3CDTF): 2015-02-03T05:25:05+09:00
• Date Created (W3CDTF): 2017-02-17
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Original Material (URI): https://dx.doi.org/10.1267/ahc.14018
• Periodical Title (URI): http://hdl.handle.net/10422/8931; https://shiga-med.repo.nii.ac.jp/records/2865
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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