Bone augmentation by highly porous β-TCP scaffold coated with PLGA and FGF2

• Material Type: 博士論文
• Title: Bone augmentation by highly porous β-TCP scaffold coated with PLGA and FGF2
• Author/Editor: 吉田, 崇
• Publication Date: 2014-03-25
• Publication Date (W3CDTF): 2014-03-25
• Alternative Title: 高気孔性PLGA /β-TCPスキャフォールドとFGF2による骨増生効果
• Degree Grantor: Hokkaido University（北海道大学）
• Date Granted: 2014-03-25
• Date Granted (W3CDTF): 2014-03-25
• Dissertation Number: 甲第11263号
• Degree Type: 博士(歯学)
• Conferring No. (Dissertation): 10101甲第11263号
• Text Language Code: eng
• Subject Heading: β-tricalcium phosphate ( β - TCP ); co-poly lactic acid /glycolic acid (PLGA); fibroblast growth factor-2 (FGF2); bone tissue engineering; regenerative scaffold
• NDC: 497
• Note (General): β-tricalcium phosphate (β-TCP), a bio-absorbable ceramic, facilitates bone conductivity. We constructed a highly porous three dimensional scaffold using β-TCP for bone tissue engineering and coated it with co-poly lactic acid/glycolic acid (PLGA) and fibroblast growth factor-2 (FGF2) to improve the mechanical strength and biological performance. The aim of this study was to examine the effect of the PLGA/FGF2-coated β-TCP scaffold on bone augmentation. The β-TCP scaffold was fabricated by the replica method using polyurethane foam, then coated with PLGA. The PLGA/β-TCP scaffold was characterized by SEM, TEM, XRD, compressive testing, cell culture and a subcutaneous implant test. Subsequently, the β-TCP scaffold, PLGA-coated scaffold, and β-TCP scaffold and PLGA-coated scaffold loaded with FGF2, were implanted into rat cranial bone. Histological observations were taken at 10 and 35 days post-surgery. SEM and TEM observations showed a thin PLGA layer on the β-TCP particle after coating. High porosity of the scaffold was exhibited after PLGA coating (> 90%), and the compressive strength of the PLGA/β-TCP scaffold was 6-fold greater compared to the non-coated scaffold. Good biocompatibility of the PLGA/β-TCP scaffold was found in the culture and implant tests. Histological samples from bone implanted with PLGA/β-TCP scaffold loaded with FGF2 showed significant bone augmentation, suggesting that FGF2-loaded PLGA/β-TCP scaffold was expected for bone tissue engineering.; Hokkaido University（北海道大学）. 博士(歯学)
• DOI: info:doi/10.14943/doctoral.k11263; https://doi.org/info:doi/10.14943/doctoral.k11263
• Persistent ID (NDL): info:ndljp/pid/9207474; https://dl.ndl.go.jp/pid/9207474
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2015-04-01T13:27:13+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://hdl.handle.net/2115/58257
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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2015-05-01 再収集