Propofol-induced relaxation of rat aorta is altered by aging 3-4

• Material Type: 博士論文
• ISSN: 1343-1420
• Title: Propofol-induced relaxation of rat aorta is altered by aging
• Volume: 61 3-4
• Author/Editor: Sakai, Yoko; Kawahito, Shinji; Takaishi, Kazumi; Mita, Naoji; Kinoshita, Hiroyuki; Hatakeyama, Noboru; Azma, Toshiharu; Nakaya, Yutaka; Kitahata, Hiroshi
• Author Heading: Sakai, Yoko; Kawahito, Shinji; Takaishi, Kazumi; Mita, Naoji; Kinoshita, Hiroyuki; Hatakeyama, Noboru; Azma, Toshiharu; Nakaya, Yutaka; Kitahata, Hiroshi
• Publication Date: 2014-08
• Publication Date (W3CDTF): 2014-08
• Alternative Title: プロポフォールによるラット大動脈の弛緩性は年齢により減弱する; Propofol-induced vasodilation and aging
• Periodical title: The journal of medical investigation : JMI
• Degree grantor/type: 徳島大学
• Date Granted: 2015-02-26
• Date Granted (W3CDTF): 2015-02-26
• Dissertation Number: 甲第2791号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 甲第2791号
• Text Language Code: eng
• Subject Heading: propofol; vasorelaxation; rat aorta; aging; endothelium
• Target Audience: 一般
• Note (General): Background : Propofol causes vasodilation via endothelium-dependent and -independent mechanisms. Because endothelial function is impaired with aging, the effects of propofol on endothelium-dependent vasodilation might be altered by aging. The aim of this study was thus to determine the effects of aging on vascular responses to propofol. Methods : Young (4-6 weeks old) or adult (16-25 weeks old) rats were anesthetized with sevoflurane. The thoracic aorta was dissected and cut into pieces 3-4mm in length. In some rings, the endothelium was deliberately removed. The ring segment of the aorta was mounted for isometric force recording at a resting tension of 0.5-1.0 g in a 2 ml organ bath, containing Krebs-Ringer bicarbonate buffer. Arteries were precontracted with phenylephrine, and the function of endothelium was confirmed with acetylcholine. Then, we studied the concentration-dependent effects of propofol in endothelium-intact (control group) and -denuded aortic rings (denuded group), as well as those treated with N[ω]-nitro-L-arginine methylester (L-NAME group). Results : Relaxation due to propofol was observed in the control groups of both young and adult rats in a concentration-dependent manner, but the magnitude of relaxation was significantly greater in young rats. In addition, in young rats, relaxation due to propofol was significantly and equally reduced in both L-NAME and denuded groups at all propofol concentrations that we studied (10[-6]-10[-3] M). In adult rats, relaxation due to propofol was quite similar between control and L-NAME groups at all propofol concentrations, whereas it was significantly reduced in the denuded group. Conclusion : These results suggest that endothelium-derived nitric oxide plays an important role in propofol-induced vasodilation in young rats, but not in adult rats.
• DOI: info:doi/10.2152/jmi.61.278; https://doi.org/info:doi/10.2152/jmi.61.278
• Persistent ID (NDL): info:ndljp/pid/9374931; https://dl.ndl.go.jp/pid/9374931
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2015-06-02T13:06:44+09:00
• Format (IMT): application/pdf
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• Periodical Title (URI): http://repo.lib.tokushima-u.ac.jp/109571
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