Flavones inhibit LPS-induced atrogin-1/MAFbx expression in mouse C2C12 skeletal myotubes

• Material Type: 博士論文
• ISSN: 1881-7742
• Title: Flavones inhibit LPS-induced atrogin-1/MAFbx expression in mouse C2C12 skeletal myotubes
• Volume: 61 2
• Author/Editor: Shiota, Chieko; Abe, Tomoki; Kawai, Nobuhiko; Ohno, Ayako; Teshima-Kondo, Shigetada; Mori, Hiroyo; Terao, Junji; Tanaka, Eiji; Nikawa, Takeshi
• Author Heading: Shiota, Chieko; Abe, Tomoki; Kawai, Nobuhiko; Ohno, Ayako; Teshima-Kondo, Shigetada; Mori, Hiroyo; Terao, Junji; Tanaka, Eiji; Nikawa, Takeshi
• Publication, Distribution, etc.: The Vitamin Society of Japan|Japan Society of Nutrition and Food Science|Center for Academic Publications Japan
• Publication Date: 2015-06-08
• Publication Date (W3CDTF): 2015-06-08
• Alternative Title: C2C12筋管細胞においてフラボンはLPS誘導性atrogin-1/MAFbxの発現を抑制する; Flavones Prevent LPS-Induced Muscle Atrophy
• Periodical title: Journal of Nutritional Science and Vitaminology
• Degree grantor/type: 徳島大学
• Date Granted: 2015-03-23
• Date Granted (W3CDTF): 2015-03-23
• Dissertation Number: 甲第2813号
• Degree Type: 博士（歯学）
• Conferring No. (Dissertation): 甲第2813号
• Text Language Code: eng
• Subject Heading: flavones; LPS; muscle atrophy; JNK
• Target Audience: 一般
• Note (General): Muscle atrophy is a complex process that occurs as a consequence of various stress events. Muscle atrophy-associated genes (atrogenes) such as atrogin-1/MAFbx and MuRF-1 are induced early in the atrophy process, and the increase in their expression precedes the loss of muscle weight. Although antioxidative nutrients suppress atrogene expression in skeletal muscle cells, the inhibitory effects of flavonoids on inflammation-induced atrogin-1/MAFbx expression have not been clarified. Here, we investigated the inhibitory effects of flavonoids on lipopolysaccharide (LPS)-induced atrogin-1/MAFbx expression. We examined whether nine flavonoids belonging to six flavonoid categories inhibited atrogin-1/MAFbx expression in mouse C2C12 myotubes. Two major flavones, apigenin and luteolin, displayed potent inhibitory effects on atrogin-1/MAFbx expression. The pretreatment with apigenin and luteolin significantly prevented the decrease in C2C12 myotube diameter caused by LPS stimulation. Importantly, the pretreatment of LPS-stimulated myoblasts with these flavones significantly inhibited LPS-induced JNK phosphorylation in C2C12 myotubes, resulting in the significant suppression of atrogin-1/MAFbx promoter activity. These results suggest that apigenin and luteolin, prevent LPS-mediated atrogin-1/MAFbx expression through the inhibition of the JNK signaling pathway in C2C12 myotubes. Thus, these flavones, apigenin and luteolin, may be promising agents to prevent LPS-induced muscle atrophy.
• DOI: info:doi/10.3177/jnsv.61.188; https://doi.org/info:doi/10.3177/jnsv.61.188
• Persistent ID (NDL): info:ndljp/pid/9425025; https://dl.ndl.go.jp/pid/9425025
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2015-07-01T13:17:09+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
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• Periodical Title (URI): http://repo.lib.tokushima-u.ac.jp/109398
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp