Novel assay to detect increased level of neutralizing anti-interferon gamma autoantibodies in non-tuberculous mycobacterial patients

• Material Type: 博士論文
• Title: Novel assay to detect increased level of neutralizing anti-interferon gamma autoantibodies in non-tuberculous mycobacterial patients
• Author/Editor: Shima, Kenjiro
• Publication, Distribution, etc.: 新潟大学
• Publication Date: 2015-03-23
• Publication Date (W3CDTF): 2015-03-23
• Alternative Title: Novel assay to detect increased level of neutralizing anti-interferon gamma autoantibodies in non-tuberculous mycobacterial patients; 非結核性抗酸菌症患者における抗インターフェロンγ中和自己抗体検出法
• Degree grantor/type: 新潟大学
• Date Granted: 2015-03-23
• Date Granted (W3CDTF): 2015-03-23
• Dissertation Number: 甲第3989号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 甲第3989号
• Text Language Code: eng
• Subject Heading: anti interferon gamma autoantibodies; disseminated non-tuberculous mycobacterium infection; Signal Transducers and Activator of Transcription 1; phosphorylation; diagnosis
• Note (General): Subjects exposed to non-tuberculouse mycobacterium (NTM) species do not always develop an active disease, which likely reflects underlying host susceptibility factors. Recent reports have shown that anti interferon gamma (IFN-γ) neutralizing autoantibodies (IFN-γAb) are associated with the development of disseminated NTM in patients without known evidence of immunodeficiency. The purpose of this study is to establish the screening method if subjects have IFN-γ Ab. Whole blood was obtained from patients with disseminated NTM, those with pulmonary NYM, and healthy controls. The neutralizing capacity to IFN-γ activity was assessed as an inhibition of Signal Transducer and Activation of Transcription 1 (STAT-1) phosphorylation in leukocyte after stimulation with exogenous IFN-γ by flow cytometer. The strength of phosphorylation was described as STAT1 phosphorylation index. Antigen capture assay was performed to measure the relative titer of Immunoglobulin-G fraction of IFN-γ Ab. STAT1 phosphorylation by IFN-γ was significantly inhibited in the leukocytes from patients with disseminated NTM compared to that in healthy subjects, while this inhibition was not observed in patients with pulmonary NYM. All subjects with inhibited STAT1 phosphorylation had high titer of Immunoglobulin-G that reacted with IFN-γ in the antigen capture assay. The measurement of STAT1 phosphorylation index in whole blood leukocytes and antigen capture assay are simple and useful method for detection of anti- IFN-γ neutralizing autoantibodies, and is valuable in the pathophysiological diagnosis of disseminated NTM patients without obvious immunodeficiency.; 学位の種類: 博士（医学）. 報告番号: 甲第3989号. 学位記番号: 新大院博（医）甲第635号. 学位授与年月日: 平成27年3月23日; Journal of Infection and Chemotherapy. 2014, 20(1), 52-56.; 新大院博（医）甲第635号; 元資料の権利情報 : Copyright (C) 2013 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/; 開始ページ : 1; 終了ページ : 24
• DOI: info:doi/10.1016/j.jiac.2013.08.003; https://doi.org/info:doi/10.1016/j.jiac.2013.08.003
• Persistent ID (NDL): info:ndljp/pid/9425147; https://dl.ndl.go.jp/pid/9425147
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2015-07-01T13:17:09+09:00
• Date Created (W3CDTF): 2019-08-05
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://hdl.handle.net/10191/32273
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp