Biological impacts of resveratrol, quercetin, and N-acetylcysteine on oxidative stress in human gingival fibroblasts

• Material Type: 博士論文
• ISSN: 1880-5086
• Title: Biological impacts of resveratrol, quercetin, and N-acetylcysteine on oxidative stress in human gingival fibroblasts
• Volume: 56 3
• Author/Editor: Orihuela Campos, Rita Cristina; Tamaki, Naofumi; Mukai, Rie; Fukui, Makoto; Miki, Kaname; Terao, Junji; Ito, Hiro-O
• Author Heading: Orihuela Campos, Rita Cristina; Tamaki, Naofumi; Mukai, Rie; Fukui, Makoto; Miki, Kaname; Terao, Junji; Ito, Hiro-O
• Publication, Distribution, etc.: SOCIETY FOR FREE RADICAL RESEARCH JAPAN
• Publication Date: 2015-05-01
• Publication Date (W3CDTF): 2015-05-01
• Alternative Title: ヒト歯肉線維芽細胞における酸化ストレスに対するレスベラトロール，ケルセチン，及びN-アセチルシステインの生物学的影響
• Periodical title: Journal of Clinical Biochemistry and Nutrition
• Degree grantor/type: 徳島大学
• Date Granted: 2015-03-23
• Date Granted (W3CDTF): 2015-03-23
• Dissertation Number: 甲第2810号
• Degree Type: 博士（歯学）
• Conferring No. (Dissertation): 甲第2810号
• Text Language Code: eng
• Subject Heading: human gingival fibroblasts; periodontitis; resveratrol; quercetin; N-acetylcysteine
• Target Audience: 一般
• Note (General): In periodontitis, production of reactive oxygen species (ROS) by neutrophils induces oxidative stress and deteriorates surrounding tissues. Antioxidants reduce damage caused by ROS and are used to treat diseases involving oxidative stress. This study summarizes the different effects of resveratrol, quercetin, and N-acetylcysteine (NAC) on human gingival fibroblasts (HGFs) under oxidative stress induced by hydrogen peroxide. Real-time cytotoxicity analyses reveals that resveratrol and quercetin enhanced cell proliferation even under oxidative stress. Of the antioxidants tested, resveratrol is the most effective at inhibiting ROS production. HGFs incubated with resveratrol and quercetin up-regulate the transcription of type I collagen gene after 3 h, but only resveratrol sustained this up-regulation for 24 h. A measurement of the oxygen consumption rate (OCR, mitochondrial respiration) shows that resveratrol generates the highest maximal respiratory capacity, followed by quercetin and NAC. Simultaneous measurement of OCR and the extracellular acidification rate (non-mitochondrial respiration) reveals that resveratrol and quercetin induce an increase in mitochondrial respiration when compared with untreated cells. NAC treatment consumes less oxygen and enhances more non-mitochondrial respiration. In conclusion, resveratrol is the most effective antioxidant in terms of real-time cytotoxicity analysis, reduction of ROS production, and enhancement of type I collagen synthesis and mitochondrial respiration in HGFs.
• DOI: info:doi/10.3164/jcbn.14-129; https://doi.org/info:doi/10.3164/jcbn.14-129
• Persistent ID (NDL): info:ndljp/pid/9479112; https://dl.ndl.go.jp/pid/9479112
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2015-08-01T14:40:15+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://repo.lib.tokushima-u.ac.jp/109399
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp