Fibrillin-1 and fibrillin-2 are essential for formation of thick oxytalan fibers in human nonpigmented ciliary epithelial cells in vitro.

• Material Type: 博士論文
• Title: Fibrillin-1 and fibrillin-2 are essential for formation of thick oxytalan fibers in human nonpigmented ciliary epithelial cells in vitro.
• Author/Editor: Yamanouchi, Kaori; Tsuruga, Eichi; Oka, Kyoko; Sawa, Yoshihiko; Ishikawa, Hiroyuki
• Author Heading: Yamanouchi, Kaori; Tsuruga, Eichi; Oka, Kyoko; Sawa, Yoshihiko; Ishikawa, Hiroyuki
• Publication Date: 2014-03-15
• Publication Date (W3CDTF): 2014-03-15
• Alternative Title: Fibrillin-1 and fibrillin-2 are essential for formation of thick oxytalan fibers in human nonpigmented ciliary epithelial cells in vitro.
• Degree grantor/type: 福岡歯科大学
• Date Granted: 2014-03-15
• Date Granted (W3CDTF): 2014-03-15
• Dissertation Number: 甲第255号
• Degree Type: 博士（歯学）
• Conferring No. (Dissertation): 甲第255号
• Text Language Code: eng
• Subject Heading: Fibrillin-1; Fibrillin-2; microfibrils; oxytalan fibers; ciliary zonule; Cells, Cultured; Ciliary Body; Epithelial Cells; Extracellular Matrix; Extracellular Matrix Proteins; Gene Expression Regulation; Gene Knockdown Techniques; Humans; Microfilament Proteins; Pigmentation; RNA, Small Interfering
• Target Audience: 一般
• Note (General): The ciliary zonule, also known as Zinn's zonule, is composed of oxytalan fibers. However, the mechanism by which epithelial cells in the ciliary body form these fibers in not fully understood. We examined human nonpigmented ciliary epithelial cells to determine the appearance and amount of oxytalan fibers in terms of positivity for their major components, fibrillin-1 and fibrillin-2. Examination of fibrillin-1 and fibrillin-2 expression by immunofluorescence revealed that thin fibers positive for fibrillin-1 on Day 2 changed to thick fibers by Day 8. The fibers positive for fibrillin-2 appeared on the thick fibrillin-1-positive fibers after Day 4. Northern blot analysis revealed that the level of fibrillin-1 did not change markedly, while induction of fibrillin-2 gene was evident on Day 5. Western blot analysis showed that fibrillin-1 deposition increased gradually, while that of fibrillin-2 increased markedly from Day 5 to Day 8. Fibrillin-1 suppression did not lead to the formation of fibrillin-2-positive thick fibers, whereas fibrillin-2 suppression led to the formation of fibrillin-1-positive thin fibers, but not thick fibers. These results suggest that both fibrillin-1 and fibrillin-2 are essential for the formation of thick oxytalan fibers in the ciliary zonule and are informative for clarifying the mechanism of homeostasis of the ocular matrix.; Connect Tissue Res. 2012;53(1):14-20. doi: 10.3109/03008207.2011.602767. Epub 2011 Aug 18.; informa
• Persistent ID (NDL): info:ndljp/pid/9479189; https://dl.ndl.go.jp/pid/9479189
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2015-08-01T14:40:15+09:00
• Date Created (W3CDTF): 2014-04-28
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Original Material (URI): Connect Tissue Res. 2012;53(1):14-20. doi: 10.3109/03008207.2011.602767. Epub 2011 Aug 18.; http://informahealthcare.com/doi/abs/10.3109/03008207.2011.602767
• Periodical Title (URI): https://fdc.repo.nii.ac.jp/records/11
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

Table of Contents

2023-08-05 再収集

2023-08-05 再収集

2023-08-05 再収集

2023-12-06 再収集

2023-12-06 再収集