p38 Mitogen-activated protein kinase and c-Jun NH2-terminal protein kinase regulate the accumulation of a tight junction protein, ZO-1, in cell-cell contacts in HaCaT cells.

• Material Type: 博士論文
• Title: p38 Mitogen-activated protein kinase and c-Jun NH2-terminal protein kinase regulate the accumulation of a tight junction protein, ZO-1, in cell-cell contacts in HaCaT cells.
• Author/Editor: Minakami, Masahiko; Kitagawa, Norio; Iida, Hiroshi; Anan, Hisashi; Inai, Tetsuichiro
• Author Heading: Minakami, Masahiko; Kitagawa, Norio; Iida, Hiroshi; Anan, Hisashi; Inai, Tetsuichiro
• Publication Date: 2015-03-14
• Publication Date (W3CDTF): 2015-03-14
• Alternative Title: p38 Mitogen-activated protein kinase and c-Jun NH2-terminal protein kinase regulate the accumulation of a tight junction protein, ZO-1, in cell-cell contacts in HaCaT cells.
• Degree grantor/type: 福岡歯科大学
• Date Granted: 2015-03-14
• Date Granted (W3CDTF): 2015-03-14
• Dissertation Number: 甲第268号
• Degree Type: 博士（歯学）
• Conferring No. (Dissertation): 甲第268号
• Text Language Code: eng
• Subject Heading: MAPK; JNK; p38; タイト結合; ZO-1; HaCaT細胞; mitogen-activated protein kinase (MAPK); c-Jun NH2-terminal protein kinase (JNK); p38 MAPK; tight junction; HaCaT cell
• Target Audience: 一般
• Note (General): To investigate the involvement of stress-activated protein kinases, JNK and p38 MAPK, in the assembly of tight junctions in keratinocytes, we treated HaCaT cells with various combinations of SP600125 (an inhibitor of JNK), SB202190 (an inhibitor of p38 MAPK) and anisomycin (an activator of both JNK and p38 MAPK) and examined the localization of ZO-1, an undercoat constitutive protein of the tight junction. Short-term (8h) incubation with SP600125, SB202190 or anisomycin induced the accumulation of ZO-1 in the cell-cell contacts, with reduced ZO-1 staining in the cytoplasm, while only long-term (24h) incubation with SP600125 induced the accumulation of ZO-1. SP600125, SB202190 or SP600125 plus SB202190 treatment induced thin linear staining for ZO-1 in the cell-cell contacts. Anisomycin treatment induced thick and irregular linear staining for ZO-1, while anisomycin plus SP600125 treatment induced zipper-like staining for ZO-1. Anisomycin plus SB202190 treatment or anisomycin plus both SP600125 and SB202190 treatment for 8h failed to lead to the accumulation of ZO-1 in cell-cell contacts, but induced thin linear staining with several gaps 16h after removal of these agents. These results suggest that the localization of ZO-1 in cell-cell contacts is differently regulated by activation and inhibition of JNK and/or p38 MAPK depending on the incubation period.; Tissue Cell. 2015 Feb;47(1):1-9. doi: 10.1016/j.tice.2014.10.001. Epub 2014 Oct 14.
• Persistent ID (NDL): info:ndljp/pid/9479205; https://dl.ndl.go.jp/pid/9479205
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2015-08-01T14:40:15+09:00
• Date Created (W3CDTF): 2015-03-16
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Original Material (URI): Tissue Cell. 2015 Feb;47(1):1-9. doi: 10.1016/j.tice.2014.10.001. Epub 2014 Oct 14.; http://dx.doi.org/10.1016/j.tice.2014.10.001
• Periodical Title (URI): https://fdc.repo.nii.ac.jp/records/33
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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