Integrin α6β4 and TRPV1 channel coordinately regulate directional keratinocyte migration.

• Material Type: 博士論文
• Title: Integrin α6β4 and TRPV1 channel coordinately regulate directional keratinocyte migration.
• Author/Editor: Miyazaki, Ayako; Ohkubo, Tsuyako; Hatta, Mitsutoki; Ishikawa, Hiroyuki; Yamazaki, Jun
• Author Heading: Miyazaki, Ayako; Ohkubo, Tsuyako; Hatta, Mitsutoki; Ishikawa, Hiroyuki; Yamazaki, Jun
• Publication Date: 2015-03-14
• Publication Date (W3CDTF): 2015-03-14
• Alternative Title: Integrin α6β4 and TRPV1 channel coordinately regulate directional keratinocyte migration.
• Degree grantor/type: 福岡歯科大学
• Date Granted: 2015-03-14
• Date Granted (W3CDTF): 2015-03-14
• Dissertation Number: 甲第267号
• Degree Type: 博士（歯学）
• Conferring No. (Dissertation): 甲第267号
• Text Language Code: eng
• Subject Heading: TRPV1チャネル; キーワード2; インテグリン beta4; 細胞遊走; TRPV1 channels; Calcium signal; Integrin beta4; Cell migration; Animals; Calcium; Capsaicin; Cell Movement; Cells, Cultured; Cobalt; Epidermal Growth Factor; Gene Silencing; Integrin alpha6beta4; Keratinocytes; Mice; TRPV Cation Channels; Up-Regulation; Wound Healing
• Target Audience: 一般
• Note (General): The directional migration of epithelial cells is crucial for wound healing. Among integrins, a family of cell adhesion receptors, integrin β4 has been assumed to be a promigratory factor, in addition to its role in stable adhesion. In turn, Ca(2+) signaling is also a key coordinator of migration. Keratinocytes reportedly express transient receptor potential vanilloid channels (TRPV1); however, the function of these channels as a regulator of intracellular Ca(2+) level in cell migration has remained uncharacterized. In the present study, we investigated the role of TRPV1 in directional migration related to integrin β4 using a scratch wound assay on a confluent monolayer sheet of murine keratinocytes (Pam212 cells). Double immunofluorescence staining revealed the de novo expression of integrin β4 and TRPV1 in migrating cells at the wound edge in response to scratch wounding, and both expression levels were almost matched. Epidermal growth factor (EGF) not only promoted keratinocyte migration, but also caused the further up-regulation of both integrin β4 and TRPV1. In addition, the knockdown of the integrin β4 or TRPV1 gene significantly impeded wound closure. The TRPV1 agonist capsaicin significantly promoted migration, while a selective TRPV1 antagonist inhibited it. The gene knockdown of TRPV1 inhibited the expression of the integrin β4 gene and that of β4 protein in migrating cells. These findings suggest that TRPV1 may stimulate directional migration directly by eliciting a Ca(2+) signal or indirectly via integrin β4 expression.; Biochem Biophys Res Commun. 2015 Feb 27;458(1):161-7. doi: 10.1016/j.bbrc.2015.01.086. Epub 2015 Jan 27.; ScienceDirect
• Persistent ID (NDL): info:ndljp/pid/9479215; https://dl.ndl.go.jp/pid/9479215
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2015-08-01T14:40:15+09:00
• Date Created (W3CDTF): 2015-07-06
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Original Material (URI): Biochem Biophys Res Commun. 2015 Feb 27;458(1):161-7. doi: 10.1016/j.bbrc.2015.01.086. Epub 2015 Jan 27.; http://authors.elsevier.com/sd/article/S0006291X15001163
• Periodical Title (URI): https://fdc.repo.nii.ac.jp/records/43
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

Table of Contents

2023-12-07 再収集

2023-12-07 再収集

2023-12-07 再収集

2024-01-29 再収集

2024-01-29 再収集