NF-κB-regulated transcriptional control of CLCA in a differentiated mouse keratinocyte line.

• Material Type: 博士論文
• Title: NF-κB-regulated transcriptional control of CLCA in a differentiated mouse keratinocyte line.
• Author/Editor: Hiromatsu, Ryo; Hatta, Mitsutoki; Okamura, Kazuhiko; Sakagami, Ryuji; Yamazaki, Jun
• Author Heading: Hiromatsu, Ryo; Hatta, Mitsutoki; Okamura, Kazuhiko; Sakagami, Ryuji; Yamazaki, Jun
• Publication Date: 2015-03-14
• Publication Date (W3CDTF): 2015-03-14
• Alternative Title: NF-κB-regulated transcriptional control of CLCA in a differentiated mouse keratinocyte line.
• Degree grantor/type: 福岡歯科大学
• Date Granted: 2015-03-14
• Date Granted (W3CDTF): 2015-03-14
• Dissertation Number: 甲第270号
• Degree Type: 博士（歯学）
• Conferring No. (Dissertation): 甲第270号
• Text Language Code: eng
• Subject Heading: 転写制御; ケラチノサイト; CLCA; NF-kB; transcriptioal control; keratinocyte
• Target Audience: 一般
• Note (General): BACKGROUND:CLCA was postulated to be a calcium-activated chloride channel accessory protein. Recent reports indicate that CLCA isoforms are likely to be expressed in different layers of the stratified epithelium of the skin.OBJECTIVE:The present study investigated the transcriptional mechanism by which murine CLCA2 (mCLCA2) is expressed in the transformed keratinocyte line Pam212 that can differentiate.METHODS:A luciferase reporter assay, chromatin immunoprecipitation (ChIP) assay, reverse transcription-PCR, and immunocytochemistry were performed using Pam212 cells.RESULTS:Promoter activity of mCLCA2 was inhibited profoundly by site-directed mutagenesis of a putative nuclear factor-κB (NF-κB) binding site and by treatment with siRNA against p65. ChIP and transcription factor assays showed the specific association of endogenously activated p65 protein with the NF-κB binding domain. As confirmed by the nuclear translocation of p65, tumor necrosis factor α and caffeic acid phenethyl ester (CAPE) increased and decreased mCLCA2 promoter activity, respectively, but exhibited modest effects on endogenous mCLCA2 expression in cells in culture medium containing 0.05 mM Ca(2+). When the Ca(2+) concentration was raised to 1.0mM, the mRNA and protein levels of mCLCA2 increased as well as those of the differentiation markers keratin 1 (K1) and K10. CAPE profoundly suppressed only the Ca(2+)-triggered expression of mCLCA2, not K1 or K10. Immunohistochemistry of native skin and organotypic 3D cultures confirmed the distribution of the CLCA2 homolog in differentiated cells.CONCLUSION:The present study revealed for the first time that basal NF-κB activity is involved in the Ca(2+)-dependent regulation of mCLCA2 expression in a mouse keratinocyte line.; J Dermatol Sci. 2015 Jun;78(3):189-96. doi: 10.1016/j.jdermsci.2015.03.007. Epub 2015 Mar 18.; ScienceDirect
• Persistent ID (NDL): info:ndljp/pid/9479429; https://dl.ndl.go.jp/pid/9479429
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2015-08-01T14:40:15+09:00
• Date Created (W3CDTF): 2015-07-22
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Original Material (URI): J Dermatol Sci. 2015 Jun;78(3):189-96. doi: 10.1016/j.jdermsci.2015.03.007. Epub 2015 Mar 18.; http://www.sciencedirect.com/science/article/pii/S0923181115000857
• Periodical Title (URI): https://fdc.repo.nii.ac.jp/records/47
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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