Matrix metalloproteinase-2 degrades fibrillin-1 and fibrillin-2 of oxytalan fibers in the human eye and periodontal ligaments in vitro.

• Material Type: 博士論文
• Title: Matrix metalloproteinase-2 degrades fibrillin-1 and fibrillin-2 of oxytalan fibers in the human eye and periodontal ligaments in vitro.
• Author/Editor: Kawagoe, Megumi; Tsuruga, Eichi; Oka, Kyoko; Sawa, Yoshihiko; Ishikawa, Hiroyuki
• Author Heading: Kawagoe, Megumi; Tsuruga, Eichi; Oka, Kyoko; Sawa, Yoshihiko; Ishikawa, Hiroyuki
• Publication Date: 2015-03-14
• Publication Date (W3CDTF): 2015-03-14
• Alternative Title: Matrix metalloproteinase-2 degrades fibrillin-1 and fibrillin-2 of oxytalan fibers in the human eye and periodontal ligaments in vitro.
• Degree grantor/type: 福岡歯科大学
• Date Granted: 2015-03-14
• Date Granted (W3CDTF): 2015-03-14
• Dissertation Number: 甲第258号
• Degree Type: 博士（歯学）
• Conferring No. (Dissertation): 甲第258号
• Text Language Code: eng
• Subject Heading: fibrillin; microfibrils; oxytalan fibers; ciliary zonule
• Target Audience: 一般
• Note (General): Oxytalan fibers are distributed in the eye and periodontal ligaments (PDL). The ciliary zonule, known as Zinn's zonule, in the eye is composed of oxytalan fibers, which are bundles of microfibrils consisting mainly of fibrillin-1 and fibrillin-2. As turnover of oxytalan fibers is slow during life, their degradation mechanism remains unclarified. This study was performed to examine degradation pattern of fibrillin-1 and fibrillin-2 by experimental MMP activation. We cultured human non-pigmented ciliary epithelial cells (HNPCEC) and PDL fibroblasts for 7 days, then treated them with concanavalin A to activate matrix metalloproteinase (MMP)-2, and examined the degradation of fibrillin-1 and fibrillin-2 for 72 hr using immunofluorescence. At 7 days of HNPCEC culture, fibrillin-1-positive fibers were observed, some of which merged with fibrillin-2. After MMP-2 activation, fibrillin-1-positive fibers became thin and disappeared by 72 hr, while fibrillin-2-positive fibers disappeared almost completely within 24 hr. At 7 days of PDL fibroblast culture, fibrillin-1-positive fibers were mostly merged with fibrillin-2. After MMP-2 activation, fibrillin-1-positive fibers became thin by 24 hr and had almost disappeared by 48 hr, while fibrillin-2-positive fibers decreased constantly after 24 hr. A MMP-2 inhibitor completely suppressed these degradations. These results suggest that the patterns of fibrillin-1 and fibrillin-2 degradation differ between the eye and the PDL, possibly reflecting the sensitivity of fibrillin-1 and fibrillin-2 of each type of oxytalan fiber against MMP-2.; Acta Histochem Cytochem. 2013 Oct 30;46(5):153-9. doi: 10.1267/ahc.13024. Epub 2013 Oct 23.; J-STAGE
• Persistent ID (NDL): info:ndljp/pid/9479431; https://dl.ndl.go.jp/pid/9479431
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2015-08-01T14:40:15+09:00
• Date Created (W3CDTF): 2015-07-23
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Original Material (URI): Acta Histochem Cytochem. 2013 Oct 30;46(5):153-9. doi: 10.1267/ahc.13024. Epub 2013 Oct 23.; https://www.jstage.jst.go.jp/article/ahc/46/5/46_13024/_article
• Periodical Title (URI): https://fdc.repo.nii.ac.jp/records/49
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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