Improved quality of cartilage repair by bone marrow mesenchymal stem cells for treatment of an osteochondral defect in a cynomolgus macaque model.

• Material Type: 博士論文
• ISSN: 1745-3674
• Title: Improved quality of cartilage repair by bone marrow mesenchymal stem cells for treatment of an osteochondral defect in a cynomolgus macaque model.
• Volume: 86 1
• Author/Editor: ARAKI, Susumu
• Author Heading: ARAKI, Susumu
• Publication, Distribution, etc.: Informa Healthcare
• Publication Date: 2015-02
• Publication Date (W3CDTF): 2015-02
• Periodical title: Acta Orthopaedica
• Degree grantor/type: 滋賀医科大学
• Date Granted: 2014-09-10
• Date Granted (W3CDTF): 2014-09-10
• Dissertation Number: 乙第410号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 乙第410号
• Text Language Code: eng
• Target Audience: 一般
• Note (General): Background and purpose:Integration of repaired cartilage with surrounding native cartilage is a major challenge for successful tissue-engineering strategies of cartilage repair. We investigated whether incorporation of mesenchymal stem cells (MSCs) into the collagen scaffold improves integration and repair of cartilage defects in a cynomolgus macaque model.; Methods:Cynomolgus macaque bone marrow-derived MSCs were isolated and incorporated into type-I collagen gel. Full-thickness osteochondral defects (3 mm in diameter, 5 mm in depth) were created in the patellar groove of 36 knees of 18 macaques and were either left untreated (null group, n = 12), had collagen gel alone inserted (gel group, n = 12), or had collagen gel incorporating MSCs inserted (MSC group, n = 12). After 6, 12, and 24 weeks, the cartilage integration and tissue response were evaluated macroscopically and histologically (4 null, 4 gel, and 4 MSC knees at each time point).; Results:The gel group showed most cartilage-rich reparative tissue covering the defect, owing to formation of excessive cartilage extruding though the insufficient subchondral bone. Despite the fact that a lower amount of new cartilage was produced, the MSC group had better-quality cartilage with regular surface, seamless integration with neighboring naïve cartilage, and reconstruction of trabecular subchondral bone.; Interpretation:Even with intensive investigation, MSC-based cell therapy has not yet been established in experimental cartilage repair. Our model using cynomolgus macaques had optimized conditions, and the method using MSCs is superior to other experimental settings, allowing the possibility that the procedure might be introduced to future clinical practice.; Thesis or Dissertation; 平成26年度; 10.3109/17453674.2014.958807
• DOI: info:doi/10.3109/17453674.2014.958807; https://doi.org/info:doi/10.3109/17453674.2014.958807
• Persistent ID (NDL): info:ndljp/pid/9479450; https://dl.ndl.go.jp/pid/9479450
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2015-08-01T14:40:15+09:00
• Date Created (W3CDTF): 2017-02-17
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://hdl.handle.net/10422/10491; https://shiga-med.repo.nii.ac.jp/records/1370
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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