Metformin impairs growth of endometrial cancer cells via cell cycle arrest and concomitant autophagy and apoptosis.

• Material Type: 博士論文
• ISSN: 1475-2867
• Title: Metformin impairs growth of endometrial cancer cells via cell cycle arrest and concomitant autophagy and apoptosis.
• Volume: 14 1
• Author/Editor: TAKAHASHI, Akimasa
• Author Heading: TAKAHASHI, Akimasa
• Publication, Distribution, etc.: BioMed Central
• Publication Date: 2014-07-16
• Publication Date (W3CDTF): 2014-07-16
• Periodical title: Cancer cell international
• Degree grantor/type: 滋賀医科大学
• Date Granted: 2015-03-10
• Date Granted (W3CDTF): 2015-03-10
• Dissertation Number: 甲第730号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 甲第730号
• Text Language Code: eng
• Subject Heading: Metformin; Endometrial cancer; Autophagy; Apoptosis
• Target Audience: 一般
• Note (General): Background:Effective therapies for early endometrial cancer usually involve surgical excision and consequent infertility Therefore, new treatment approaches that preserve fertility should be developed. Metformin, a well-tolerated anti-diabetic drug, can inhibit cancer cell growth. However, the mechanism of metformin action is not well understood. Here we investigate the roles of autophagy and apoptosis in the anti-cancer effects of metformin on endometrial cancer cells.; Methods:Ishikawa endometrial cancer cells were treated with metformin. WST-8 assays, colony formation assays, flow cytometry, caspase luminescence measurement, immunofluorescence, and western blots were used to assess the effects of metformin on cell viability, proliferation, cell cycle progression, apoptosis, and autophagy.; Results:Metformin-treated cells exhibited significantly lower viability and proliferation and significantly more cell cycle arrest in G1 and G2/M than control cells. These cells also exhibited significantly more apoptosis via both intrinsic and extrinsic pathways. In addition, metformin treatment induced autophagy. Inhibition of autophagy, either by Beclin1 knockdown or by 3-methyladenine-mediated inhibition of caspase-3/7, suppressed the anti-proliferative effects of metformin on endometrial cancer cells. These findings indicate that the anti-proliferative effects and apoptosis caused by metformin are partially or completely dependent on autophagy.; Conclusions:We showed that metformin suppresses endometrial cancer cell growth via cell cycle arrest and concomitant autophagy and apoptosis.; Thesis or Dissertation; 平成26年度; 10.1186/1475-2867-14-53
• DOI: info:doi/10.1186/1475-2867-14-53; https://doi.org/info:doi/10.1186/1475-2867-14-53
• Persistent ID (NDL): info:ndljp/pid/9479453; https://dl.ndl.go.jp/pid/9479453
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2015-08-01T14:40:15+09:00
• Date Created (W3CDTF): 2017-02-17
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://hdl.handle.net/10422/10572; https://shiga-med.repo.nii.ac.jp/records/2891
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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