Functional deficiency of MHC class I enhances LTP and abolishes LTD in the nucleus accumbens of mice

• Material Type: 博士論文
• ISSN: 19326203
• Title: Functional deficiency of MHC class I enhances LTP and abolishes LTD in the nucleus accumbens of mice
• Author/Editor: 枝村, 光浩
• Author Heading: 枝村, 光浩
• Publication, Distribution, etc.: PLOS (Public Library of Science)
• Publication Date: 2014-09-30
• Publication Date (W3CDTF): 2014-09-30
• Alternative Title: 主要組織適合遺伝子複合体クラスＩの機能欠損はマウスの側坐核のLTPを亢進しLTDを消失する
• Periodical title: PLoS ONE
• Degree grantor/type: 浜松医科大学
• Date Granted: 2014-12-12
• Date Granted (W3CDTF): 2014-12-12
• Dissertation Number: 甲第688号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 13802甲第688号
• Text Language Code: eng
• Target Audience: 一般
• Note (General): doctoral; 医学系研究科; Major histocompatibility complex class I (MHCI) molecules were recently identified as novel regulators of synaptic plasticity. These molecules are expressed in various brain areas, especially in regions undergoing activity-dependent synaptic plasticity, but their role in the nucleus accumbens (NAc) is unknown. In this study, we investigated the effects of genetic disruption of MHCI function, through deletion of β2-microblobulin which causes lack of cell surface expression of MHCI. First, we confirmed that MHCI molecules are expressed in the NAc core in wild-type mice. Second, we performed electrophysiological recordings with NAc core slices from wild-type and β2-microglobulin knock-out mice lacking cell surface expression of MHCI. We found that low frequency stimulation induced long-term depression in wild-type but not knock-out mice, whereas high frequency stimulation induced long-term potentiation in both genotypes, with a larger magnitude in knock-out mice. Furthermore, we demonstrated that knock-out mice showed more persistent behavioral sensitization to cocaine, which is a NAc-related behavior. Using this model, we analyzed the density of total AMPA receptors and their subunits GluR1 and GluR2 in the NAc core, by SDS-digested freeze-fracture replica labeling. After repeated cocaine exposure, the density of GluR1 was increased, but there was no change in total AMPA receptors and GluR2 levels in wild-type mice. In contrast, following repeated cocaine exposure, increased densities of total AMPA receptors, GluR1 and GluR2 were observed in knock-out mice. These results indicate that functional deficiency of MHCI enhances synaptic potentiation, induced by electrical and pharmacological stimulation.
• DOI: 10.1371/journal.pone.0107099; https://doi.org/10.1371/journal.pone.0107099
• Persistent ID (NDL): info:ndljp/pid/9491468; https://dl.ndl.go.jp/pid/9491468
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2015-09-01T13:12:47+09:00
• Date Created (W3CDTF): 2018-08-27
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://hdl.handle.net/10271/2878; https://hama-med.repo.nii.ac.jp/records/2744
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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