Potent tumor tropism of induced pluripotent stem cells and induced pluripotent stem cell-derived neural stem cells in the mouse intracerebral glioma model

• Material Type: 博士論文
• Title: Potent tumor tropism of induced pluripotent stem cells and induced pluripotent stem cell-derived neural stem cells in the mouse intracerebral glioma model
• Author/Editor: 山添, 知宏
• Author Heading: 山添, 知宏
• Publication, Distribution, etc.: Spandidos Publications
• Publication Date: 2015-01
• Publication Date (W3CDTF): 2015-01
• Alternative Title: iPS細胞およびiPS細胞由来神経幹細胞はマウス脳内グリオーマモデルにおいて腫瘍への活発な遊走能を有する
• Periodical title: International Journal of Oncology
• Degree grantor/type: 浜松医科大学
• Date Granted: 2015-02-11
• Date Granted (W3CDTF): 2015-02-11
• Dissertation Number: 甲第689号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 甲第689号
• Text Language Code: eng
• Subject Heading: induced pluripotent stem cells; neural stem cells; glioma; migration; gene therapy
• Target Audience: 一般
• Note (General): doctoral; 医学系研究科; Abstract. Although neural and mesenchymal stem cells have been well-known to have a strong glioma tropism, this activity in induced pluripotent stem cells (iPSCs) has not yet been fully studied. In the present study, we tested tumor tropic activity of mouse iPSCs and neural stem cells derived from the iPSC (iPS-NSCs) using in vitro Matrigel invasion chamber assay and in vivo mouse intracranial tumor model. Both iPSC and iPS-NSC had a similar potent in vitro tropism for glioma conditioned media. The migrated iPSCs to the gliomas kept expressing Nanog-GFP gene, suggesting no neuronal or glial differentiation. iPSCs or iPS-NSCs labeled with 5-bromo-2-deoxyuridine were intracranially implanted in the contralateral hemisphere to the GL261 glioma cell implantation in the allogeneic C57BL/6 mouse. Active migration of both stem cells was observed 7 days after implantation. Again, the iPSCs located in the tumor area expressed Nanog-GFP gene, suggesting that the migrated cells were still iPSCs. These findings demonstrated that both iPSCs and iPS-NSCs had potent glioma tropism and could be candidates as vehicles in stem cell-based glioma therapy.
• DOI: info:doi/10.3892/ijo.2014.2702; https://doi.org/info:doi/10.3892/ijo.2014.2702
• Persistent ID (NDL): info:ndljp/pid/9491469; https://dl.ndl.go.jp/pid/9491469
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2015-09-01T13:12:47+09:00
• Date Created (W3CDTF): 2018-08-27
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://hdl.handle.net/10271/2879; https://hama-med.repo.nii.ac.jp/records/2745
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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