Relationship between sodium-dependent phosphate transporter (NaPi-IIc) function and cellular vacuole formation in opossum kidney cells

• Material Type: 博士論文
• ISSN: 1349-6867
• Title: Relationship between sodium-dependent phosphate transporter (NaPi-IIc) function and cellular vacuole formation in opossum kidney cells
• Volume: 62 3-4
• Author/Editor: Shiozaki, Yuji; Segawa, Hiroko; Ohnishi, Saori; Ohi, Akiko; Ito, Mikiko; Kaneko, Ichiro; Kido, Shinsuke; Tatsumi, Sawako; Miyamoto, Ken-ichi
• Author Heading: Shiozaki, Yuji; Segawa, Hiroko; Ohnishi, Saori; Ohi, Akiko; Ito, Mikiko; Kaneko, Ichiro; Kido, Shinsuke; Tatsumi, Sawako; Miyamoto, Ken-ichi
• Publication, Distribution, etc.: Faculty of Medicine Tokushima University
• Publication Date: 2015-08
• Publication Date (W3CDTF): 2015-08
• Alternative Title: フクロネズミ腎近位尿細管細胞におけるナトリウム依存性リン酸トランスポーターNaPi-IIcと細胞内vacuole形成の関連; NaPi-IIc induces vacuole formation in OK cells
• Periodical title: The Journal of Medical Investigation
• Degree grantor/type: 徳島大学
• Date Granted: 2015-07-23
• Date Granted (W3CDTF): 2015-07-23
• Dissertation Number: 甲第2867号
• Degree Type: 博士（栄養学）
• Conferring No. (Dissertation): 甲第2867号
• Text Language Code: eng
• Subject Heading: proximal tubule; SLC34A3; opossum kidney cells; proliferation; vacuole
• Target Audience: 一般
• Note (General): NaPi-IIc/SLC34A3 is a sodium-dependent inorganic phosphate (Pi) transporter in the renal proximal tubules and its mutations cause hereditary hypophosphatemic rickets with hypercalciuria (HHRH). In the present study, we created a specific antibody for opossum SLC34A3, NaPi-IIc (oNaPi-IIc), and analyzed its localization and regulation in opossum kidney cells (a tissue culture model of proximal tubular cells). Immunoreactive oNaPi-IIc protein levels increased during the proliferative phase and decreased during differentiation. Moreover, stimulating cell growth upregulated oNaPi-IIc protein levels, whereas suppressing cell proliferation downregulated oNaPi-IIc protein levels. Immunocytochemistry revealed that endogenous and exogenous oNaPi-IIc proteins localized at the protrusion of the plasma membrane, which is a phosphatidylinositol 4,5-bisphosphate (PIP2) rich-membrane, and at the intracellular vacuolar membrane. Exogenous NaPi-IIc also induced cellular vacuoles and localized in the plasma membrane. The ability to form vacuoles is specific to electroneutral NaPi-IIc, and not electrogenic NaPi-IIa or NaPi-IIb. In addition, mutations of NaPi-IIc (S138F and R468W) in HHRH did not cause cellular PIP2-rich vacuoles. In conclusion, our data anticipate that NaPi-IIc may regulate PIP2 production at the plasma membrane and cellular vesicle formation.
• DOI: info:doi/10.2152/jmi.62.209; https://doi.org/info:doi/10.2152/jmi.62.209
• Persistent ID (NDL): info:ndljp/pid/9529602; https://dl.ndl.go.jp/pid/9529602
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2015-11-01T14:53:18+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://repo.lib.tokushima-u.ac.jp/111281
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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2019-09-06 再収集