Possible selection of viable human blastocysts after vitrification by monitoring morphological changes

• Material Type: 博士論文
• Title: Possible selection of viable human blastocysts after vitrification by monitoring morphological changes
• Author/Editor: Maezawa, Tadashi
• Author Heading: Maezawa, Tadashi
• Publication, Distribution, etc.: 三重大学
• Publication Date: 2015-01-01
• Publication Date (W3CDTF): 2015-01-01
• Degree grantor/type: 三重大学
• Date Granted: 2015-03-25
• Date Granted (W3CDTF): 2015-03-25
• Dissertation Number: 甲医学第1705号
• Degree Type: 博士(医学)
• Conferring No. (Dissertation): 甲医学第1705号
• Text Language Code: eng
• Alias of Author: マエザワ, タダシ; 前沢, 忠志
• Subject Heading: Vitrified-warmed blastocyst; Oxygen consumption; Time-lapse images; Embryo selection; Embryo transfer
• Target Audience: 一般
• Note (General): application/pdf; Morphological assessment of human blastocysts has been effective for selecting embryos with high potential. However, they often show repeated shrinkage and expansion toward their hatching. Here we assessed whether capturing morphological changes over time of vitrified-warmed blastocysts could lead to a better selection of viable embryos from shrunken blastocysts. The implantation rates of vitrified-warmed blastocysts that were shrunken or expanded (developing) at the time of loading for transfer were compared among 2,729 cycles that were subjected to single blastocyst transfer. Vitrified (107) and fresh blastocysts (17) were donated for the experimental study. To assess the relationship between morphology (expanded vs. shrunken) and the mitochondrial respiration of blastocysts, the oxygen consumption rate (OCR) was analyzed for 55 specimens using an uncoupler of oxidative phosphorylation. The remaining 69 blastocysts were used for recording morphological changes every 15 min for 48 h after warming. Because there were no surplus embryos, 7 % of the vitrified-warmed blastocysts were shrunken and transferred. The shrunken embryos had sufficient implantation ability (40 %). The OCR of the shrunken embryos was significantly lower than that of their expanded counterparts. Upon exposure to the uncoupler, the OCR of some shrunken embryos increased to levels similar to the expanded specimens. Time-lapse images revealed some shrunken embryos which formed blastocoel by 5 h following warming exhibited developmental competence to the hatched stage. Data of the present study suggest a group of shrunken blastocysts contains many viable and clinically available embryos and time-lapse observation of vitrified-warmed blastocysts is a potential method to distinguish viable embryos from shrunken blastocysts.; 本文 / Mie Univ. ; IVF Namba Clin, Osaka ; IVF Osaka Clinic, Higashi-Osaka; 8p; http://link.springer.com/article/10.1007%2Fs10815-014-0260-x
• DOI: info:doi/10.1007/s10815-014-0260-x; https://doi.org/info:doi/10.1007/s10815-014-0260-x
• Persistent ID (NDL): info:ndljp/pid/9530066; https://dl.ndl.go.jp/pid/9530066
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2015-11-01T14:53:18+09:00
• Date Created (W3CDTF): 2017-02-18
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Related Material (URI): http://link.springer.com/article/10.1007%2Fs10815-014-0260-x
• Periodical Title (URI): http://hdl.handle.net/10076/14726; https://mie-u.repo.nii.ac.jp/records/7833
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

Table of Contents

2017-04-02 再収集

2017-08-02 再収集

2023-12-15 再収集