A high ratio of G1 to G0 phase cells and an accumulation of G1 phase cells before S phase progression after injurious stimuli in the proximal tubule

• Material Type: 博士論文
• Title: A high ratio of G1 to G0 phase cells and an accumulation of G1 phase cells before S phase progression after injurious stimuli in the proximal tubule
• Volume: 2
• Author/Editor: Iwakura, Takamasa
• Author Heading: Iwakura, Takamasa
• Publication, Distribution, etc.: John Wiley and Sons
• Publication Date: 2014
• Publication Date (W3CDTF): 2014
• Alternative Title: 近位尿細管での高いG1/G0期細胞比率と傷害刺激後におけるS期進行前のG1期細胞蓄積
• Periodical title: Physiological Reports
• Degree grantor/type: 浜松医科大学
• Date Granted: 2015-03-16
• Date Granted (W3CDTF): 2015-03-16
• Dissertation Number: 甲第705号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 甲第705号
• Text Language Code: eng
• Subject Heading: Cell cycle; G0-G1 transition; G1 arrest; proximal tubule.
• Target Audience: 一般
• Note (General): doctoral; 医学系研究科; Proximal tubule (PT) cells can proliferate explosively after injurious stimuli. To investigate this proliferative capacity, we examined cell cycle status and the expression of cyclin-dependent kinase inhibitor p27, a G1 phase mediator, in PT cells after a proliferative or injurious stimulus. Rats were treated with lead acetate (proliferative stimulus) or uranyl acetate (UA; injurious stimulus). Isolated tubular cells were separated into PT and distal tubule (DT) cells by density-gradient centrifugation. Cell cycle status was analyzed with flow cytometry by using the Hoechst 33342/pyronin Y method. Most PT and DT cells from control rats were in G0/G1 phase, with a higher percentage of PT cells than DT cells in G1 phase. Lead acetate and UA administration promoted the G0-G1 transition and the accumulation of G1 phase cells before S phase progression. In PT cells from rats treated with lead acetate or a subnephrotoxic dose of UA, p27 levels increased or did not change, possibly reflecting G1 arrest. In contrast, p27 became undetectable before the appearance of apoptotic cells in rats treated with a nephrotoxic dose of UA. The decrease in p27 might facilitate rapid cell cycling. The decreased number of p27-positive cells was associated with PT cell proliferation in renal tissues after a proliferative or injurious stimulus. The findings suggest that a high ratio of G1 to G0 phase cells and a rapid accumulation of G1 phase cells before S phase progression in the PT is a biological strategy for safe, timely, and explosive cell proliferation in response to injurious stimuli.
• DOI: info:doi/10.14814/phy2.12173; https://doi.org/info:doi/10.14814/phy2.12173
• Persistent ID (NDL): info:ndljp/pid/9550895; https://dl.ndl.go.jp/pid/9550895
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2015-12-01T13:36:39+09:00
• Date Created (W3CDTF): 2018-08-27
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://hdl.handle.net/10271/2907; https://hama-med.repo.nii.ac.jp/records/2768
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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