Initial Transient Accumulation of M2 Macrophage-associated Molecule-expressing Cells after Pulpotomy with Mineral Trioxide Aggregate in Rat Molars

• Material Type: 博士論文
• Title: Initial Transient Accumulation of M2 Macrophage-associated Molecule-expressing Cells after Pulpotomy with Mineral Trioxide Aggregate in Rat Molars
• Author/Editor: Takei, Erika
• Publication, Distribution, etc.: 新潟大学
• Publication Date: 2015-03-23
• Publication Date (W3CDTF): 2015-03-23
• Alternative Title: Initial Transient Accumulation of M2 Macrophage-associated Molecule-expressing Cells after Pulpotomy with Mineral Trioxide Aggregate in Rat Molars; Mineral Trioxide Aggregateを用いた生活断髄後のラット臼歯歯髄の反応 : M2マクロファージ関連分子陽性細胞の一過性集積
• Degree Grantor: 新潟大学
• Date Granted: 2015-03-23
• Date Granted (W3CDTF): 2015-03-23
• Dissertation Number: 甲第4003号
• Degree Type: 博士（歯学）
• Conferring No. (Dissertation): 甲第4003号
• Text Language Code: eng
• Subject Heading: CD68; CD163; CD204; dental pulp; macrophage; mineral trioxide aggregate; pulpotomy; rat (Wistar)
• Note (General): Introduction: M2 (alternatively-activated) macrophages are known to participate in wound healing and tissue repair. This study aimed to analyze the temporospatial changes in the distribution and density of M2 macrophage-associated molecule-expressing cells after pulpotomy with mineral trioxide aggregate (MTA) in rat molars, in order to ascertain the role played by M2 macrophages in the healing of MTA-capped pulp tissue. Methods: The maxillary first molars of 8-week-old Wistar rats were pulpotomized and capped with MTA. After 1-14 days, the teeth were examined after hematoxylin-eosin staining or immunoperoxidase staining of CD68 (a general macrophage marker) and M2 macrophage markers (CD163 and CD204). The density of positively-stained cells was enumerated in the surface and inner regions (0 -100 µm and 300-400 µm, respectively, from the wound surface). Results: MTA-capping initially caused mild inflammatory changes and the formation of a degenerative layer, followed by progressive new matrix formation and calcified bridging. At 1-2 days, CD68-, CD163-, and CD204-positive cells started to accumulate beneath the degenerative layer, and the density of these cells was significantly higher in the surface region than in the inner region (P < 0.05). From 7 days onwards, the three types of cells displayed an almost normal distribution beneath the newly dentin-like matrix. Conclusions: After the pulpotomy of rat molars with MTA, M2 macrophage-associated molecule-expressing cells transiently accumulated beneath the degenerative layer under the MTA. This suggests that M2 macrophages participate in the initial phases of the healing of MTA-capped pulp tissue.; 学位の種類: 博士（歯学）. 報告番号: 甲第4003号. 学位記番号: 新大院博（歯）甲第316号. 学位授与年月日: 平成27年3月23日; Journal of endodontics. 2014, 40(12), 1983-1988.; 新大院博（歯）甲第316号; 元資料の権利情報 : Copyright(C) 2014 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.; 開始ページ : 1; 終了ページ : 25
• DOI: info:doi/10.1016/j.joen.2014.08.012; https://doi.org/info:doi/10.1016/j.joen.2014.08.012
• Persistent ID (NDL): info:ndljp/pid/9577632; https://dl.ndl.go.jp/pid/9577632
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2016-01-05T14:58:43+09:00
• Date Created (W3CDTF): 2019-08-05
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://hdl.handle.net/10191/32172
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp