Activation of NLRP3 inflammasome in dendritic cells and macrophages by Mycoplasma salivarium

• Material Type: 博士論文
• Title: Activation of NLRP3 inflammasome in dendritic cells and macrophages by Mycoplasma salivarium
• Author/Editor: 杉山, 正博
• Author Heading: 杉山, 正博
• Publication, Distribution, etc.: Hokkaido University
• Publication Date: 2015-03-25
• Publication Date (W3CDTF): 2015-03-25
• Alternative Title: Mycoplasma salivariumによる樹状細胞ならびにマクロファージにおけるNLRP3インフラマソーム活性化
• Contributor: 北川, 善政; 柴田, 健一郎; 田村, 正人
• Degree grantor/type: 北海道大学
• Date Granted: 2015-03-25
• Date Granted (W3CDTF): 2015-03-25
• Dissertation Number: 甲第11729号
• Degree Type: 博士(歯学)
• Conferring No. (Dissertation): 甲第11729号
• Text Language Code: eng
• Alias of Author: Kang, Sung-Sik
• NDC: 497
• Target Audience: 一般
• Note (General): The proinflammatory cytokine IL-1β plays crucial roles in the pathogenesis ofperiodontal disease. IL-1β is produced after processing of pro-IL-1β by caspase-1,which is activated by the multiprotein complex consisting of NLR, an adaptor proteinand procaspase-1, called an the inflammasome. Mycoplasma salivarium, human oralmicrobial flora, preferentially inhabits the gingival sulcus and is considered to play anetiological role in periodontal diseases. As a first step to clarify the etiological role ofthe organism in periodontal diseases, this study was designed to clarify whether itinduces production of IL-1β by innate immune cells such as dendritic cells ormacrophages by using M. pneumoniae, which is known to induce production of IL-1βby human macrophages, as a positive control.It was found that both live and heat-killed cells of M. salivarium induced productionof IL-1β by the murine dendritic cell line XS106 cells and also necrotic cell death calledpyroptosis in the cells. The activity was significantly and partially downregulated bysilencing of caspase-1 and NLRP3, respectively. BMMs from wild-type and NLRP3-,ASC- and caspase-1-deficient mice were examined for IL-1β production in response tothe mycoplasma. Live cells of M. salivarium almost completely lost the activity toinduce IL-1β production by macrophages derived from ASC- and caspase-1-deficientmice. In addition, the activity toward BMMs from NLRP3-deficient mice wassignificantly but not completely attenuated. These results suggest that live cells of M.salivarium are able to activate several types of inflammasome, including the NLRP3inflammasome, to produce IL-1β.Taken together, the results of this study suggests that M. salivarium plays anetiological role in periodontal diseases through induction of inflammatory IL-1βproduction by dendritic cells and macrophages that have infiltrated inflamed gingivalconnective tissue.; (主査) 教授 北川 善政, 教授 柴田 健一郎, 教授 田村 正人; 歯学研究科（口腔医学専攻）
• DOI: 10.14943/doctoral.k11729; https://doi.org/10.14943/doctoral.k11729
• Persistent ID (NDL): info:ndljp/pid/9577796; https://dl.ndl.go.jp/pid/9577796
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2016-01-05T14:58:43+09:00
• Date Created (W3CDTF): 2015-03
• Format (IMT): PDF
• Access Restrictions: 国立国会図書館内限定公開
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• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://dx.doi.org/10.14943/doctoral.k11729; http://hdl.handle.net/2115/60276
• Is Referenced By (URI): http://hdl.handle.net/2115/59249
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp