好熱菌由来SODの精製および，その活性と構造に関する研究

• Material Type: 博士論文
• Title: 好熱菌由来SODの精製および，その活性と構造に関する研究
• Author/Editor: 丁, 凌云
• Author Heading: 丁, 凌云
• Publication Date: 2015-03-23
• Publication Date (W3CDTF): 2015-03-23
• Degree grantor/type: 室蘭工業大学
• Date Granted: 2015-03-23
• Date Granted (W3CDTF): 2015-03-23
• Dissertation Number: 甲第375号
• Degree Type: 博士（工学）
• Conferring No. (Dissertation): 甲第375号
• Text Language Code: jpn
• Alias of Author: DING, LING YUN
• NDC: 579
• Target Audience: 一般
• Note (General): application/pdf; Reactive oxygen species such as super oxide anion was produced as a by-product from aerobic respiration metabolism. Because superoxide dismutase (SOD) extinguishes the active oxygen or oxide anion radical, it plays a role in DNA and enzymes protecting against oxygen damage. SOD is a universal antioxidant enzyme in a microbe, a higher animal, a plant. The SOD has a Cu/Zn type, a Mn type, and a Fe type by the species that there is in the active center, and the MnSOD exists both in eukaryote and prokaryote. Thermophilic bacteria can grow under high temperature (more than 55 degrees Celsius). This paper has described purification of MnSOD which is derived from thermophilic bacteria C36 strain by gel chromatography under the presence of surfactant SDS. This new method was achieved the high recovery of SOD to be approximately 33%. It would be able to get a SOD with tolerance (SDS tolerance, heat resistance, pH tolerance) to be the industrial usage. And this new isolation method can apply the other thermophilic bacteria, too. MnSOD has dimeric structure. However, the function of the monomer-dimer conversion of the SOD is not clarified. SODs were chemistry cross linked with divalent reagents such as glutaraldehyde to fix a dimer conformation. The activity of cross-linked SOD largely decreased because molecular structure was fixed. The inter-subunit flexibility of the SOD was shown to be important to present SOD activity.
• DOI: 10.15118/00005131; https://doi.org/10.15118/00005131
• Persistent ID (NDL): info:ndljp/pid/9921631; https://dl.ndl.go.jp/pid/9921631
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2016-04-01T14:32:40+09:00
• Date Created (W3CDTF): 2016-02-15
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://dx.doi.org/10.15118/00005131; http://hdl.handle.net/10258/3802; https://muroran-it.repo.nii.ac.jp/records/5140
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp