Decreased DNA methylation in the promoter region of the WNT5A and GDNF genes may promote the osteogenicity of mesenchymal stem cells from patients with ossified spinal ligaments.

• Material Type: 博士論文
• Title: Decreased DNA methylation in the promoter region of the WNT5A and GDNF genes may promote the osteogenicity of mesenchymal stem cells from patients with ossified spinal ligaments.
• Author/Editor: Chiba, Noriyuki
• Author Heading: Chiba, Noriyuki
• Publication Date: 2015-09-30
• Publication Date (W3CDTF): 2015-09-30
• Alternative Title: WNT5AとGDNFのプロモーター領域におけるDNAメチル化の減少によって脊柱靱帯骨化症患者由来の間葉系幹細胞の骨化が促進される; WNT5A ト GDHF ノ プロモーター リョウイキ ニオケル DNA メチルカ ノ ジンタイ コッカショウ カンジャ ユライノ カンヨウ ケイ カンサイボウ ノ コッカ ガ ソクシン サレル
• Degree grantor/type: 弘前大学
• Date Granted: 2015-09-30
• Date Granted (W3CDTF): 2015-09-30
• Dissertation Number: 甲第1903号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 甲第1903号
• Text Language Code: eng
• Subject Heading: 基礎医学
• NDC: 491.366
• Target Audience: 一般
• Note (General): type:Thesis; Mesenchymal stem cells (MSCs) isolated from spinal ligaments with ectopic ossification have a pro- pensity toward the osteogenic lineage. To explore epigenetic control of the osteogenic features of MSCs, we treated MSCs obtained from the spinal ligaments of ossification of yellow ligament (OYL) Patients and non-OYL patients with the DNA methyltransferase inhibitor, 5-aza-20-deoxycytidine (5AdC). We compared the non-OYL groups (untreated and treated with 5AdC) with the OYL groups (untreated and treated with 5AdC) by genome-wide microarray analysis. Next, we used methylated DNA immunopre- cipitation combined with quantitative real-time PCR to assess gene methylation. Ninety-eight genes showed expression significantly increased by 5AdC treatment in MSCs from non-OYL patients but not from OYL patients. In contrast, only two genes, GDNF and WNT5A, showed significantly higher expression in OYL MSCs compared with non-OYL MSCs without 5AdC treatment. Both genes were hypermethylated in non-OYL MSCs but not in OYL MSCs. Small interfering RNA targeted to each gene decreased expression of the target gene and also several osteogenic genes. Both small interfering RNAs also suppressed the activity of alkaline phosphatase, a typical marker of osteogenesis. These results suggest that the osteo- genic features of MSCs from OYL patients are promoted by unmethylated WNT5A and GDNF genes.; 掲載誌Journal of Pharmacological Sciences, Volume 127, Issue 4, April 2015, Pages 467–473http://www.sciencedirect.com/science/article/pii/S1347861315000705 doi:10.1016/j.jphs.2015.03.008
• Persistent ID (NDL): info:ndljp/pid/9968961; https://dl.ndl.go.jp/pid/9968961
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2016-05-01T16:38:40+09:00
• Date Created (W3CDTF): 2018-03-13
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://hdl.handle.net/10129/5664
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

Table of Contents

2017-11-03 再収集

2018-06-04 再収集

2018-06-04 再収集

2023-03-03 再収集

2023-03-03 再収集