Use of Cryopreserved Osteogenic Matrix Cell Sheets for Bone Reconstruction.

• Material Type: 博士論文
• ISSN: 2161-6760
• Title: Use of Cryopreserved Osteogenic Matrix Cell Sheets for Bone Reconstruction.
• Volume: 6 1
• Author/Editor: Kura, Tomohiko; Akahane, Manabu; Shimizu, Takamasa; Uchihara, Yoshinobu; Tohma, Yasuaki; Morita, Yusuke; Koizumi, Munehisa; Kawate, Kenji; Tanaka, Yasuhito
• Author Heading: Kura, Tomohiko; Akahane, Manabu; Shimizu, Takamasa; Uchihara, Yoshinobu; Tohma, Yasuaki; Morita, Yusuke; Koizumi, Munehisa; Kawate, Kenji; Tanaka, Yasuhito
• Publication, Distribution, etc.: Scientific Research Publishing Inc.
• Publication Date: 2016-01; 6 1
• Publication Date (W3CDTF): 2016-01
• Alternative Title: 凍結保存骨形成細胞シートの骨再建における有用性
• Periodical title: Stem Cell Discovery
• Degree grantor/type: 奈良県立医科大学
• Date Granted: 2016-03-15
• Date Granted (W3CDTF): 2016-03-15
• Dissertation Number: 甲第650号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 甲第650号
• Text Language Code: eng
• Subject Heading: Bone Marrow Stromal Cell; Cryopreservation; Cell Sheet; Injectable Bone; Bone Reconstruction
• Target Audience: 一般
• Note (General): type:Thesis; Skeletal diseases, such as nonunion and osteonecrosis, are now treatable with tissue engineering techniques. Single cell sheets called osteogenic matrix cell sheets (OMCSs) grown from cultured bone marrow-derived mesenchymal stem cells show high osteogenic potential; however, long preparation times currently limit their clinical application. Here, we report a cryopreservation OMCS transplantation method that shortens OMCS preparation time. Cryopreserved rat OMCSs were prepared using slow- and rapid-freezing methods, thawed, and subsequently injected scaffold-free into subcutaneous sites. Rapid- and slow-frozen OMCSs were also transplanted directly to the femur bone at sites of injury. Slow-freezing resulted in higher cell viability than rapid freezing, yet all two cryopreservation methods yielded OMCSs that survived and formed bone tissue. In the rapid- and slow-freezing groups, cortical gaps were repaired and bone continuity was observed within 6 weeks of OMCS transplantation. Moreover, while no significant difference was found in osteocalcin expression between the three experimental groups, the biomechanical strength of femurs treated with slow-frozen OMCSs was significantly greater than those of non-transplant at 6 weeks post-injury. Collectively, these data suggest that slow-frozen OMCSs have superior osteogenic potential and are better suited to produce a mineralized matrix and repair sites of bone injury.; 博士（医学）・甲第650号・平成28年3月15日; Copyright © 2016 by authors and Scientific Research Publishing Inc.This work is licensed under the Creative Commons Attribution International License (CC BY).http://creativecommons.org/licenses/by/4.0/; identifier:Stem Cell Discovery Vol.6 No.1 p.13-23(2016.01); identifier:21616760; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/3187; identifier:Stem Cell Discovery, 6(1): 13-23
• DOI: info:doi/10.4236/scd.2016.61002; https://doi.org/info:doi/10.4236/scd.2016.61002
• Persistent ID (NDL): info:ndljp/pid/9969034; https://dl.ndl.go.jp/pid/9969034
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2016-05-01T16:38:40+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://hdl.handle.net/10564/3187
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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