Identification of pregnancy-associated plasma protein A as a migration-promoting gene in malignant pleural mesothelioma cells : a potential therapeutic target.

• Material Type: 博士論文
• ISSN: 1949-2553
• Title: Identification of pregnancy-associated plasma protein A as a migration-promoting gene in malignant pleural mesothelioma cells : a potential therapeutic target.
• Volume: 4 8
• Author/Editor: Huang, Jun; Tabata, Sho; Kakiuchi, Soji; Van, Trung The; Goto, Hisatsugu; Hanibuchi, Masaki; Nishioka, Yasuhiko
• Author Heading: Huang, Jun; Tabata, Sho; Kakiuchi, Soji; Van, Trung The; Goto, Hisatsugu; Hanibuchi, Masaki; Nishioka, Yasuhiko
• Publication, Distribution, etc.: Impact Journals
• Publication Date: 2013-07-08
• Publication Date (W3CDTF): 2013-07-08
• Alternative Title: Pregnancy-associated plasma protein Aを悪性胸膜中皮腫細胞の遊走能を促進する遺伝子として同定 : 治療標的としての可能性
• Periodical title: Oncotarget
• Degree grantor/type: 徳島大学
• Date Granted: 2013-09-12
• Date Granted (W3CDTF): 2013-09-12
• Dissertation Number: 甲第2630号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 甲第2630号
• Text Language Code: eng
• Subject Heading: malignant pleural mesothelioma; pregnancy-associated plasma protein A; migration; orthotopic xenograft mouse model
• Target Audience: 一般
• Note (General): Despite recent advances in treatment, malignant pleural mesothelioma (MPM) remains a deadly disease. Targeted therapy generated broad interests and is highly expected for the treatment of MPM, yet promising preclinical results have not been translated into substantial clinical benefits for the patients. In this study, we tried to identify the genes which play functional roles in cell migration as well as to test whether they can be used as novel targets for molecular targeted therapy for MPM in preclinical model. In our study, pregnancy-associated plasma protein A (PAPPA) was identified as a gene whose expression level is correlated with MPM cell migration by correlation analysis combining MPM cell migration ability and their gene expression profiles. Highly migratory cells were selected from MPM cell lines, MSTO-211H, NCI-H290 and EHMES-1 in vitro and up-regulation of PAPPA in these cells were confirmed. In vitro, PAPPA was demonstrated to stimulate the MPM cell migration via cleavage of insulin-like growth factor-binding protein-4 and subsequent release of IGF-1. Gene silencing of PAPPA in MPM cells led to reduced migration, invasion and proliferation. Furthermore, PAPPA shRNA transfected NCI-H290 when orthotopically inoculated into pleural cavity of severe combined immunodeficiency recipient mice, failed to develop tumors and produce bloody pleural effusion as control shRNA transfected cells did. Our study suggests that PAPPA plays a functional role in promoting MPM cell migration and it might serve as a potential therapeutic target for the treatment of MPM.
• DOI: info:doi/10.18632/oncotarget.1126; https://doi.org/info:doi/10.18632/oncotarget.1126
• Persistent ID (NDL): info:ndljp/pid/9984208; https://dl.ndl.go.jp/pid/9984208
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2016-06-03T01:05:16+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://repo.lib.tokushima-u.ac.jp/105907
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp