フリッカー光刺激後の網膜血流増加反応における Glia 細胞の役割 12

• Material Type: 博士論文
• Title: フリッカー光刺激後の網膜血流増加反応における Glia 細胞の役割
• Volume: 56 12
• Author/Editor: 宋, 勇錫
• Publication Date: 2015-11
• Publication Date (W3CDTF): 2015-11
• Alternative Title: Role of Glial Cells in Regulating Retinal Blood Flow during Flicker-Induced Hyperemia in Cats
• Periodical title: Investigative Ophthalmology and Visual Science
• Degree grantor/type: 旭川医科大学
• Date Granted: 2016-03-25
• Date Granted (W3CDTF): 2016-03-25
• Dissertation Number: 甲第495号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 甲第495号
• Text Language Code: eng
• Note (General): PURPOSE: To investigate how glial cells participate in retinal circulation during flicker stimulation in cats. METHODS: Using laser Doppler velocimetry, we measured the vessel diameter and blood velocity simultaneously and calculated the retinal blood flow (RBF) in feline first-order retinal arterioles. Twenty-four hours after intravitreal injections of L-2-aminoadipic acid (LAA), a gliotoxic compound, and the solvent of 0.01 N hydrochloric acid as a control, we examined the changes in RBF in response to 16-Hz flicker stimulation for 3 minutes. We also measured the changes in RBF 2 hours after intravitreal injection of Nω-propyl-L-arginine (L-NPA), a selective neuronal nitric oxide synthase inhibitor, in LAA-treated eyes. To evaluate the effects of LAA on retinal neuronal function, ERGs were monitored. Immunohistochemical examinations were performed. RESULTS: In LAA-treated eyes, histologic changes selectively occurred in retinal glial cells. There were no significant reductions in amplitude or elongation of implicit time in ERG after LAA injections compared with controls. In control eyes, the RBF gradually increased and reached the maximal level (53.5% ± 2.5% increase from baseline) after 2 to 3 minutes of flicker stimulation. In LAA-treated eyes, the increases in RBF during flicker stimulation were attenuated significantly compared with controls. In LAA-treated eyes 2 hours after injection of L-NPA, flicker-evoked increases in RBF decreased significantly compared with LAA-treated eyes. CONCLUSIONS: The current results suggested that increases in RBF in response to flicker stimulation were regulated partly by retinal glial cells.; identifier:PMID:26618647; 開始ページ : 7551; 終了ページ : 7559
• DOI: info:doi/10.1167/iovs.15-17676; https://doi.org/info:doi/10.1167/iovs.15-17676
• Persistent ID (NDL): info:ndljp/pid/9984459; https://dl.ndl.go.jp/pid/9984459
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2016-06-03T01:05:16+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://amcor.asahikawa-med.ac.jp/modules/xoonips/detail.php?id=20160325_K495
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp