Intravital bone imaging by two-photon excitation microscopy to identify osteocytic osteolysis in vivo.
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- Material Type
- 博士論文
- Author/Editor
- Sano, Hiroshige
- Publication, Distribution, etc.
- Publication Date
- 2016-03-23
- Publication Date (W3CDTF)
- 2016-03-23
- Alternative Title
- Intravital bone imaging by two-photon excitation microscopy to identify osteocytic osteolysis in vivo.2光子励起顕微鏡を用いた骨細胞性骨溶解解明の試み
- Degree Grantor
- 新潟大学
- Date Granted
- 2016-03-23
- Date Granted (W3CDTF)
- 2016-03-23
- Dissertation Number
- 甲第4132号
- Degree Type
- 博士（医学）
- Conferring No. (Dissertation)
- 甲第4132号
- Text Language Code
- eng
- Subject Heading
- Note (General)
- Bone is a highly dynamic organ in which several cell types function cooperatively. Among these, osteocytes have recently emerged as an important regulator of bone homeostasis, although their mechanism of regulation is unclear. Here, intravital bone imaging by two-photon excitation microscopy allowed us to directly visualize ‘osteocytic osteolysis’, or resorption of bone in the lacuno-canalicular system. Osteocyte lacunae and the canalicular network in the cortex of murine tibiae were imaged by in vivo calcein staining, and local acidification in these structures was monitored using a topically applied pH sensor. We also demonstrated that sciatic neurectomy causes significant acidification around osteocytic lacunae and enlargement of lacuno-canalicular areas. These results provide strong evidence for osteocytic osteolysis, and demonstrate that two-photon intravital microscopy is useful for analysis of this phenomenon.学位の種類: 博士（医学）. 報告番号: 甲第4132号. 学位記番号: 新大院博（医）甲第699号. 学位授与年月日: 平成28年3月23日Bone. 2015, 74, 134-139.新大院博（医）甲第699号元資料の権利情報 : Copyright(C) 2015 Elsevier Inc. All rights reserved.
- DOI
- info:doi/10.1016/j.bone.2015.01.013
- Persistent ID (NDL)
- info:ndljp/pid/9984821
- Collection
- Collection (Materials For Handicapped People:1)
- Collection (particular)
- 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文
- Acquisition Basis
- 博士論文（自動収集）
- Date Accepted (W3CDTF)
- 2016-06-03T01:05:16+09:00
- Date Created (W3CDTF)
- 2019-08-05
- Format (IMT)
- application/pdf
- Access Restrictions
- 国立国会図書館内限定公開
- Service for the Digitized Contents Transmission Service
- 図書館・個人送信対象外
- Availability of remote photoduplication service
- 可
- Periodical Title (URI)
- Data Provider (Database)
- 国立国会図書館 : 国立国会図書館デジタルコレクション