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- ContentsFront Cover Series Page Methods in ENZYMOLOGY Copyright Cont...... of dihydrouridine modifications in RNA 1 Introduction 1.1 ......A function 1.2 Methods to detect dihydrouridine 2 Step-by-ste......A) selection using oligo (dT) ce......A) selection using oligo (dT) ma......ze selection using silica column......3.3 3′ End healing 3.4 RNA size ......lysis 4.1 Trimming of adapter se......plicate collapsing 4.3 UMI remov......l 4.4 Read mapping 4.5 Read end position gathering 4.6 D-site id......ion 5.2 Sequencing depth and bio......olution sequencing technology for mapping pseudouridines in mammalian mRNA......olution sequencing technology 2 Materials 2.1 P...
- Related MaterialMethods in enzymology
- Periodical TitleMethods in enzymology
Paper図書
National Diet LibraryOther Libraries in Japan
- ContentsFront Cover Series Page Methods in Enzymology Copyright Cont......ructured RNA 1 Introduction 2 General methods of RT-PCR using MarathonRT 2....... Before you begin 2.2 Key resour......ls 2.4.1 Annealing RT primers to......es 2.4.2 Preparing the reverse t......ure 2.4.3 Carrying out the rever...... 2.4.4 Programming a thermal cyc......er 2.4.5 Preparing the PCR mixture 2.4.6 Carrying out the PCR a......tion 2.4.7 Examining the PCR produ......nd troubleshooting 2.8.1 Potenti......ons for optimizing the procedure...... Sanger sequencing of RNAs containing complex RNA r......ents 2 General methods of RNA Sanger sequencing usin...
- Related MaterialMethods in enzymology
- Periodical TitleMethods in enzymology
Paper図書
National Diet Library
- Summary, etc.In this volume we......cols concentrating on Protein, carefully written and edited...
- Subject HeadingProteins--Analysis. Proteins--Purification......zymologie Proteine Purification
- Title HeadingMethods in enzymology ; v. 559.
Paper図書
National Diet Library
- Subject HeadingEnzymology.
- Note (General)Includes indexes.
- Title HeadingMethods in enzymology ; v. 582.
Paper図書
National Diet Library
- Subject HeadingCell Membrane--enzymology. Membranes--enzymology.
- Title HeadingMethods in enzymology ; v. 584.
Paper図書
National Diet Library
- Summary, etc....most 50 years, Methods in Enzymology is one of the ......d publications in the field of b......0,000 chapters in the collection......or researchers in all fields of life sciences, including microbiology,...... This volume brings together a n......cols concentrating on protein, carefully wri......ed by experts, including: Pulse-chase ......o measure protein degradationLabeling a protein with fluorophores using NHS ester der......zationImmunoaffinity purification of proteinsProteolytic affinity tag cleavag......ST-tagged proteins Indispensable too......xperts to contain step-by-step p......core protocols involving protein.
- Subject HeadingProteins--Analysis.
- Title HeadingMethods in enzymology ; v. 536.
Paper図書
National Diet Library
- ContentsChemokine detection using recetors immo...... and cell-cell interactions in cancer with mi......devices Generating chemokine analogs with ......l properties using phage display Methods for the recogn......AG-bound chemokines Monitoring scavenging activity of chemokine receptors Dua......tion for chemokine receptor signaling Analysis of arrestin recruitment to chemokine receptors by bioluminescence resonan......y transfer Probing biased signaling in chemokine receptors Mut......e display Studying chemokine control of ne......phil migration in vivo in a murine model of inflammatory arth......ction of chemokine/chemokine receptor complexes...
- Subject HeadingChemokines. Chemokines--Physiological effect.
- Title HeadingMethods in enzymology ; v. 570.
Paper図書
National Diet Library
- ContentsSection 1: Protein protocols/protein precipiation: ......ction II: Protein protocols/protein pull-down methods: Coimmunopreci......tation of proteins from yeast Coupling antibody to c......alysis of protein-protein interactions by c......tion III: Protein protocols/protein purification: ......of hydrophobic interaction chrom......raphy for protein purification H......gies for recombinant proteins Salting out of proteins using ammonium sulf......recipitation Using ion exchange ......purify a recombinantly expressed protein Gel filtration......raphy) of proteins Section IV: Protein protocols/puri...... membrane proteins: Expression and purification...
- Subject HeadingProteins--Analysis. Proteins--Purification.
- Title HeadingMethods in enzymology ; v. 541.
Paper図書
National Diet Library
- Contents...of metal ions binding to proteins Assess coupled protein folding and binding through tempe......nt-based screening for enzyme inhibitors using calorimetry Measuring multivalent binding interactions by i......uplex DNA-containing sugar and bas......n of cyclodextrin-mediated deter...... membrane-protein reconstitution Extending ITC to kinetics with KinlTC Measuring the kinetics of molecu......reactions Avoiding buffer interference in ITC experiment......ydrogenase ITC methods for assessing buffer/protein interactions from...... steady-state kinetics: a reacti......alysis of protein folding by differential scanning calorimetry A guide to diffe...
- Title HeadingMethods in enzymology ; v. 567.
Paper図書
National Diet Library
- Subject HeadingCell Membrane--enzymology. Membranes--enzymology. Membrane Proteins.
- Title HeadingMethods in enzymology ; v. 583.
Paper図書
National Diet Library
- ContentsHow to study intermediate filaments in atomic detail ...... properties of intermediate filament proteins Multidimensional monitoring of keratin intermediate filaments in cultured cells......body probes of intermediate filament proteins Assays for po......difications of intermediate filament proteins Immunofluores......ection of keratins - High-throughput screening for drugs that moderate intermediate filament proteins The use of Withaferin A to study intermediate fila......of cytoplasmic intermediate fila......epidermal keratins Using data mining and computati......aches to study intermediate fila......alysis of keratins and keratin-associated prote...
- Subject HeadingIntermediate filament proteins.
- Title HeadingMethods in enzymology ; v. 568.
Paper図書
National Diet Library
- ContentsBioID identification of lamin-associated proteins Proximity-dependent biotin identification (BioID) in Dictyostelium ......sis of LEM-domain proteins Purification ......N and KASH domain proteins - Purification and lamins and soluble f......nctionally distinct populations of lamin-binding proteins Development o......uroescent protein-based binding assay to stud......ciation of plakins with intermediate filament proteins Functional analysis of keratin-associated proteins in intestinal epithelia: heat-shock protein chaperoning and kinase rescue Puri......cation of protein chaperones and......al assays with intermediate filaments Purificat...
- Subject HeadingProteins. Intermediate filament proteins.
- Title HeadingMethods in enzymology ; v. 569.
Paper図書
National Diet Library
- ContentsFront Cover; Peptide, Protein and Enzyme Des...... Catalysts; 1. Introduction; 2. ......ugates as Protein Modification C......II) Conjugates in Organic Soluti......Metalated FKBP Inhibitor (Coughlin et al., 2014) ......II) Conjugates in Aqueous Soluti...... of an SH3 Domain and Gel-Based ......ration of Protein Modification B......Catalyzed Protein Modification; ......ion of SH3 Domain of Yes Kinase in Mammalian Cell......Vohidov, Coughlin, & Ball, 2015)......ne-Tagged Protein by Chemical Blotting3.4.1. Fluores......dified SH3 Domain; References; C......ter Two: Cell-Binding Assays for Determining the Affinity of Protein-Pr...
- Title HeadingMethods in enzymology ; v. 580.
- Author HeadingPecoraro, Vincent L.
Paper図書
National Diet Library
- Contents...rodynamic modeling and its application in AUC Calculatio......lf-association in diluted and cr......s Analysis of linked equilibria Elucidating complicated assembling systems in biology using size-and-shap......rterization of intrinsically disordered proteins by analytical......l-angle scattering for membrane proteins Hydrodynamic ......nspecific protein-DNA interactions Char......operative protein-DNA clusters b......gomeric chromatin complexes using interference detection Dissecting steroid recep......ltracentrifuge methods for the analys......gates, and lignins Analytical ul......n and its role in development and research of t...
- Title HeadingMethods in enzymology ; v. 562.
Paper図書
National Diet Library
- Contents... Northern blotting Explanatory c......tection assays In vitro transcri......Measurement of in vivo synthesis......thod for measuring mRNA decay rate in saccharomyces ......ysis for determining mRNA and ribo......me association in saccharomyces ......NA degradation intermediates Cir...... RNA radiolabeling Fluorescently labeling synthetic RNA......ntration determination RNA purif......-precipitation methods Reverse transc......dideoxy sequencing of RNA RNA st......RNA backbone using in-line probing.
- Title HeadingMethods in enzymology ; v. 530.
Paper図書
National Diet Library
- Title HeadingMethods in enzymology volume 566.
Paper図書
National Diet Library
- Genre/Form TermsConference papers and proceedings.
- Subject Heading...Congresses. Luminescent probes--......ent probes. Luminescent probes.
- Title HeadingMethods in enzymology ; v. 581.
Paper図書
National Diet Library
- Title HeadingMethods in enzymology ; v. 533.
Paper図書
National Diet Library
- Subject HeadingEpigenetics. Enzymology. Epigenomics.
- Note (General)Includes indexes.
- Title HeadingMethods in enzymology ; v. 573.
Paper図書
National Diet Library
- Contents...atory chapter: introducing exogenous DNA into cells Agaros...... southern blotting Purification ......igos by denaturing polyacrylamid...... Molecular cloning Rapid creatio......ammalian cell lines for regulate......ession of proteins using the Gateway® recombination cloning technology and Flp-In-REx® lines Restrictionless cloning Isolation of ......dideoxy sequencing of DNA Prepar......eration sequencing on the applie......eration sequencing Generating mammalian stable cell lines by electropo......h polyethylenimine (PEI) Site-di......r: troubleshooting PCR Explanatory chapter: qua...
- Title HeadingMethods in enzymology ; v. 529.