Alternative Title“油症”における免疫応答の考察：油症患者の炎症を生じやすい素因におけるNK細胞とTreg細胞の役割
Degree grantor/typeNagasaki University (長崎大学)
Note (General)Background: In 1968 in western Japan, polychlorinated biphenyl-contaminated “Kanemi rice oil” was used in cooking, causing food poisoning in many people. More than 50 years have passed since the Yusho incident, and although inflammatory disorders such as suppuration have been observed in Yusho patients, the etiology of this inflammation susceptibility remains obscure. Objectives: To investigate the mechanisms of susceptibility to inflammation in Yusho patients, peripheral immune cell fractions and concentrations of inflammatory cytokines were evaluated in blood samples collected from both Yusho patients and age-matched healthy subjects undergoing medical examination in Nagasaki. Methods: To exclude diagnostic uncertainty, serum levels of polychlorinated biphenyl (PCB), polychlorinated quarterphenyl (PCQ), and polychlorinated dibenzofuran (PCDF) were measured. Immune cell (e.g. natural killer and regulatory T cell) populations were analyzed by flow cytometry. Serum cytokines involved in immune cell activation were measured by ELISA. Results: The relative proportion of natural killer cells was higher in Yusho patients than in healthy subjects, while the proportion of regulatory T cells did not differ between groups. Serum concentrations of IL-36 and IFN-γ were significantly lower in Yusho patients than in healthy subjects. Conversely, serum cytotoxic T lymphocyte-associated antigen-4 (CTLA-4), which is a cytokine related to activated NK cells, was higher in Yusho patients than in healthy subjects and was positively correlated with PCDF blood levels. Conclusion: Increased numbers of NK cells in Yusho patients suggests that the innate immune response has been activated in Yusho patients. The seemingly paradoxical results for CTLA-4 and IFN-γ may reflect counterbalancing mechanisms preventing excessive NK cell activation. This dysregulation of innate immunity might contribute to the inflammation observed in Yusho patients.
長崎大学学位論文 学位記番号:博(医歯薬)甲第1254号 学位授与年月日:令和2年6月3日
Author: Yoshiyuki Kamio, Yumi Gunge, Yuta Koike, Yutaka Kuwatsuka, Kazuto Tsuruta, Katsunori Yanagihara, Masutaka Furue, Hiroyuki Murota
Citation: Environmental Research, 185, art.no.109415; 2020
identifier:Nagasaki University (長崎大学), 博士(医学) (2020-06-03)
http://hdl.handle.net/10069/40081
DOIinfo:doi/10.1016/j.envres.2020.109415
Collection (particular)国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文
Date Accepted (W3CDTF)2020-07-06T20:31:19+09:00
Data Provider (Database)国立国会図書館 : 国立国会図書館デジタルコレクション