Analysis of cereal extracts as conditioning solutes to suppress the initial attachment of Escherichia coli to abiotic surfaces

• Material Type: 博士論文
• ISSN: 1438-2377
• Title: Analysis of cereal extracts as conditioning solutes to suppress the initial attachment of Escherichia coli to abiotic surfaces
• Volume: 246 5
• Author/Editor: Sakai, Hitomi; Sakai, Tohru; Badr, Hoida Ali Badr; Kanemaru, Kaori; Yokoigawa, Kumio
• Author Heading: Sakai, Hitomi; Sakai, Tohru; Badr, Hoida Ali Badr; Kanemaru, Kaori; Yokoigawa, Kumio
• Publication, Distribution, etc.: Springer
• Publication Date: 2020-03-02
• Publication Date (W3CDTF): 2020-03-02
• Alternative Title: 非微生物素材表面への大腸菌の初期付着を抑制する穀類抽出液の分析
• Periodical title: European Food Research and Technology
• Degree grantor/type: 徳島大学
• Date Granted: 2020-03-12
• Date Granted (W3CDTF): 2020-03-12
• Dissertation Number: 甲第3411号
• Degree Type: 博士（栄養学）
• Conferring No. (Dissertation): 甲第3411号
• Text Language Code: eng
• Subject Heading: Cereals; Escherichia coli; Attachment; Abiotic materials
• Target Audience: 一般
• Note (General): We examined the initial attachment of E. coli to abiotic surfaces conditioned with cereal extracts. The extracts were water-soluble fractions prepared from flours of barley, quinoa, rice and wheat. Strains used were E. coli ATCC 8739, E. coli NBRC 3301, E. coli NBRC 3302, E. coli NBRC 13168, E. coli NBRC 13891, and E. coli O157:H7 sakai. When surfaces of glass and stainless steel were conditioned at 25°C for 30 min with 0.5% cereal extracts, significantly lower numbers of E. coli cells attached to the conditioned surfaces than unconditioned ones, irrespective of strains used. The highest activity in reduction of the number of E. coli cells attached to the abiotic surfaces was found in the wheat extract. The suppressive activity was stable after treatments of the extract by autoclave and enzymatic digestion with α-amylase and Proteinase K. We purified the active compound by ammonium sulfate fractionation and gel filtration with HiPrep 16/60 Sephacryl S-200 HR after the enzymatic treatments. The purified compound showed an average molecular mass of about 300 kDa by light-scattering measurements. Analyses of its components indicated that the active compound was arabinoxylan; the molar ratios were 1.0 (arabinose) to 2.46 (xylose). Commercially available arabinoxylan (average molecular mass: 370 kDa) also showed the similar activity. To our knowledge, this is the first report on a dietary fiber from cereals which suppresses the initial attachment of E. coli to abiotic surfaces.
• DOI: info:doi/10.1007/s00217-020-03444-9; https://doi.org/info:doi/10.1007/s00217-020-03444-9
• Persistent ID (NDL): info:ndljp/pid/11659958; https://dl.ndl.go.jp/pid/11659958
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2021-04-07T03:01:57+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://repo.lib.tokushima-u.ac.jp/114680
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp