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博士論文
Topographic Map of Serotonin system : a foundation study for investigating pathophysiology of Autism Spectrum Disorder
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国立国会図書館デジタルコレクション
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DOI[10.24561/00019580]to the data of the same series
Topographic Map of Serotonin system : a foundation study for investigating pathophysiology of Autism Spectrum Disorder
- Persistent ID (NDL)
- info:ndljp/pid/12313435
- Material type
- 博士論文
- Author
- JANAK, RAJ AWASTHI
- Publisher
- 埼玉大学大学院理工学研究科
- Publication date
- 2021
- Material Format
- Digital
- Capacity, size, etc.
- -
- Name of awarding university/degree
- 埼玉大学,博士(学術)
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- type:textIt is well established that serotonergic fibers distribute throughout the brain. Abnormal densities or patterns of serotonergic fibers have b...
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Digital
- Material Type
- 博士論文
- Author/Editor
- JANAK, RAJ AWASTHI
- Author Heading
- Publication, Distribution, etc.
- Publication Date
- 2021
- Publication Date (W3CDTF)
- 2021
- Alternative Title
- 自閉症の病態生理の理解にむけたセロトニン繊維走行の解明
- Periodical title
- 博士論文（埼玉大学大学院理工学研究科（博士後期課程））
- Degree grantor/type
- 埼玉大学
- Date Granted
- 2021-03-25
- Date Granted (W3CDTF)
- 2021-03-25
- Dissertation Number
- 乙第261号
- Degree Type
- 博士(学術)
- Conferring No. (Dissertation)
- 乙第261号
- Text Language Code
- eng
- Subject Heading
- Target Audience
- 一般
- Note (General)
- type:textIt is well established that serotonergic fibers distribute throughout the brain. Abnormal densities or patterns of serotonergic fibers have been implicated in neuropsychiatric disorders. Although many classical studies have examined the distribution pattern of serotonergic fibers, most of them were either limited to specific brain areas or had limitations in demonstrating the fine axonal morphology. In this study, we utilize transgenic mice expressing GFP under the serotonin transporter (SERT) promoter to map the topography of serotonergic fibers across the rostro-caudal extent of each brain area. We demonstrate previously unreported regional density and fine-grained anatomy of serotonergic fibers. Our findings include: 1) SERT fibers distribute abundantly in the thalamic nuclei close to the midline and dorsolateral areas, in most of the hypothalamic nuclei with few exceptions such as the median eminence and arcuate nuclei, and within the basal amygdaloid complex and lateral septal nuclei, 2) the source fibers of innervation of the hippocampus traverse through the septal nuclei before reaching its destination, 3) unique, filamentous type of straight terminal fibers within the nucleus accumbens, 4) laminar pattern of innervation in the hippocampus, olfactory bulb and cortex with heterogenicity in innervation density among the layers, 5) cortical labelling density gradually decreases rostro-caudally, 6) fibers traverse and distribute mostly within the gray matter, leaving the white fiber bundles uninnervated, and 7) most of the highly labelled nuclei and cortical areas have predominant anatomical connection to limbic structures. Although the precise role of serotonergic projections in all of these nuclei are yet to be fully determined, we discuss their possible functional relevance. In conclusion, we provide novel, regionally specific insights on the distribution map of serotonergic fibers using transgenic mouse.ACKNOWLEDGEMENTS ................................................................................................................................... 01. INTRODUCTION...................................................................................................................................... 42. REVIEW OF LITERATURES ....................................................................................................................... 4 2.1. HISTORY OF SEROTONIN MAPPING .......................................................................................................... 4 2.2. BIOCHEMISTRY OF SEROTONIN ............................................................................................................... 7 2.3. DIVERSITY OF SEROTONERGIC NEURONS ................................................................................................... 8 2.4. DEVELOPMENT OF 5-HT RAPHE NEURONS AND THEIR PROJECTIONS ................................................................ 9 2.5. PROJECTIONS OF RAPHE NEURONS ........................................................................................................ 10 2.6. SEROTONIN TRANSPORTER: FUNCTIONAL AND CLINICAL IMPLICATIONS ........................................................... 12 COMPREHENSIVE TOPOGRAPHIC MAP OF THE SEROTONERGIC FIBERS IN THE MOUSE BRAIN ..................... 15 ABSTRACT .................................................................................................................................................... 151. INTRODUCTION.................................................................................................................................... 162. MATERIAL AND METHODS ................................................................................................................... 17 2.1. ANIMALS ....................................................................................................................................... 17 2.2. IMMUNOHISTOCHEMISTRY ................................................................................................................. 17 2.3. IMAGES ACQUISITION AND ANALYSIS ..................................................................................................... 18 3. RESULTS ............................................................................................................................................... 18 3.1. ASCENDING 5-HT PATHWAY AND SUB-PATHWAYS IN THE ADULT BRAIN.......................................................... 19 3.1.1. SUPPLEMENTARY ASCENDING FIBER BUNDLE ....................................................................................... 20 3.2. RAPHE SYSTEM ................................................................................................................................ 20 3.3. INNERVATION PATTERN IN THE THALAMUS ............................................................................................. 22 3.3.1. MIDLINE NUCLEI .......................................................................................................................... 22 3.3.2. INTRALAMINAR NUCLEI.................................................................................................................. 22 3.3.3. ASSOCIATION NUCLEI .................................................................................................................... 23 3.3.4. PRINCIPAL NUCLEI ........................................................................................................................ 23 3.3.5. MEDIAL AND LATERAL HABENULA (EPITHALAMUS) ................................................................................ 24 3.3.6. RETICULAR NUCLEUS..................................................................................................................... 24 3.3.7. OTHER AREAS ............................................................................................................................. 24 3.4. INNERVATION PATTERN IN THE HYPOTHALAMUS ...................................................................................... 24 3.4.1. PREOPTIC REGION ........................................................................................................................ 25 3.4.2. SUPRAOPTIC REGION .................................................................................................................... 25 3.4.3. TUBERAL REGION ......................................................................................................................... 26 3.4.4. MAMILLARY REGION ..................................................................................................................... 26 3.4.5. OTHER AREAS WITHIN THE HYPOTHALAMIC ZONE ................................................................................. 27 3.5. INNERVATION PATTERN IN THE AMYGDALA ............................................................................................. 27 3.5.1. DEEP OR BASOLATERAL GROUP ........................................................................................................ 28 3.5.2. SUPERFICIAL OR CORTICAL-LIKE GROUP .............................................................................................. 28 3.5.3. CENTROMEDIAL GROUP ................................................................................................................. 28 3.5.4. OTHER NUCLEI ............................................................................................................................ 29 3.6. INNERVATION PATTERN IN THE SEPTUM ................................................................................................. 29 3.6.1. THE NUCLEUS OF DIAGONAL BAND (NBD) AND MEDIAL SEPTUM (MS) ...................................................... 29 3.6.2. LATERAL SEPTAL NUCLEUS .............................................................................................................. 30 3.6.3. OTHER SEPTAL AREAS ................................................................................................................... 31 3.7. INNERVATION PATTERN IN THE BASAL GANGLIA ....................................................................................... 31 3.8. INNERVATION PATTERN IN THE NUCLEUS ACCUMBENS .............................................................................. 32 3.9. INNERVATION PATTERN IN THE BED NUCLEUS OF STRIA TERMINALIS ............................................................. 32 3.10. INNERVATION PATTERN IN THE HIPPOCAMPUS......................................................................................... 32 3.11. INNERVATION PATTERN IN THE CORTEX ................................................................................................. 33 3.11.1. PREFRONTAL CORTEX ................................................................................................................... 34 3.11.2. RETROSPLENIAL CORTEX ................................................................................................................ 34 3.11.3. MOTOR CORTEX .......................................................................................................................... 35 3.11.4. SOMATOSENSORY CORTEX ............................................................................................................. 35 3.11.5. AUDITORY AND VISUAL CORTEXES .................................................................................................... 35 3.11.6. RHINAL AREA ............................................................................................................................. 36 3.11.7. PIRIFORM CORTEX........................................................................................................................ 36 3.12. INNERVATION PATTERN IN THE OLFACTORY TUBERCLE AND OLFACTORY BULB ................................................. 36 3.13. INNERVATION PATTERN IN THE CEREBELLUM ........................................................................................... 374. DISCUSSION ......................................................................................................................................... 37 4.1. THALAMUS ..................................................................................................................................... 39 4.2. HYPOTHALAMUS .............................................................................................................................. 40 4.3. AMYGDALA..................................................................................................................................... 42 4.4. BASAL GANGLIA ............................................................................................................................... 43 4.5. CORTEX ......................................................................................................................................... 445. CONCLUSION ....................................................................................................................................... 45DEVELOPMENT OF SEROTONERGIC PROJECTIONS TO SUPRACHIASMATIC NUCLEUS IN THE MOUSE BRAIN . 64ABSTRACT ..................................................................................................................................................... 641. INTRODUCTION.................................................................................................................................... 652. MATERIALS AND METHOD ................................................................................................................... 66 2.1. ANIMALS ....................................................................................................................................... 66 2.2. IMMUNOHISTOCHEMISTRY ................................................................................................................. 66 2.3. IMAGES ACQUISITION AND ANALYSIS ..................................................................................................... 663. RESULTS ............................................................................................................................................... 674. DISCUSSION ......................................................................................................................................... 685. CONCLUSION ....................................................................................................................................... 69SCOPE OF THE STUDY .................................................................................................................................... 70REFERENCES .................................................................................................................................................. 73指導教員 : SHIMOGORI Tomomi
- DOI
- 10.24561/00019580
- Persistent ID (NDL)
- info:ndljp/pid/12313435
- Collection
- Collection (Materials For Handicapped People:1)
- Collection (particular)
- 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文
- Acquisition Basis
- 博士論文（自動収集）
- Date Accepted (W3CDTF)
- 2022-08-08T06:02:54+09:00
- Date Created (W3CDTF)
- 2022-06-15
- Format (IMT)
- application/pdf
- Access Restrictions
- 国立国会図書館内限定公開
- Service for the Digitized Contents Transmission Service
- 図書館・個人送信対象外
- Availability of remote photoduplication service
- 可
- Periodical Title (URI)
- Data Provider (Database)
- 国立国会図書館 : 国立国会図書館デジタルコレクション